PMID- 25754368
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20200930
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 207
IP  - 3
DP  - 2015 Aug
TI  - A novel intracellular nitrogen-fixing symbiosis made by Ustilago maydis and 
      Bacillus spp.
PG  - 769-77
LID - 10.1111/nph.13359 [doi]
AB  - We observed that the maize pathogenic fungus Ustilago maydis grew in nitrogen 
      (N)-free media at a rate similar to that observed in media containing ammonium 
      nitrate, suggesting that it was able to fix atmospheric N2 . Because only 
      prokaryotic organisms have the capacity to reduce N2 , we entertained the 
      possibility that U. maydis was associated with an intracellular bacterium. The 
      presence of nitrogenase in the fungus was analyzed by acetylene reduction, and 
      capacity to fix N2 by use of (15) N2 . Presence of an intracellular N2 -fixing 
      bacterium was analyzed by PCR amplification of bacterial 16S rRNA and nifH genes, 
      and by microscopic observations. Nitrogenase activity and (15) N incorporation 
      into the cells proved that U. maydis fixed N2 . Light and electron microscopy, 
      and fluorescence in situ hybridization (FISH) experiments revealed the presence 
      of intracellular bacteria related to Bacillus pumilus, as evidenced by sequencing 
      of the PCR-amplified fragments. These observations reveal for the first time the 
      existence of an endosymbiotic N2 -fixing association involving a fungus and a 
      bacterium.
CI  - (c) 2015 The Authors New Phytologist (c) 2015 New Phytologist Trust.
FAU - Ruiz-Herrera, Jose
AU  - Ruiz-Herrera J
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Irapuato, Guanajuato, 
      Mexico.
FAU - Leon-Ramirez, Claudia
AU  - Leon-Ramirez C
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Irapuato, Guanajuato, 
      Mexico.
FAU - Vera-Nunez, Antonio
AU  - Vera-Nunez A
AD  - Departamento de Biotecnologia y Bioquimica, Unidad Irapuato, Centro de 
      Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, 
      Irapuato, Guanajuato, Mexico.
FAU - Sanchez-Arreguin, Alejandro
AU  - Sanchez-Arreguin A
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Irapuato, Guanajuato, 
      Mexico.
FAU - Ruiz-Medrano, Roberto
AU  - Ruiz-Medrano R
AD  - Departamento de Biotecnologia y Bioingenieria, Unidad Zacatenco, Centro de 
      Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Mexico 
      City, Mexico.
FAU - Salgado-Lugo, Holjes
AU  - Salgado-Lugo H
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Irapuato, Guanajuato, 
      Mexico.
FAU - Sanchez-Segura, Lino
AU  - Sanchez-Segura L
AD  - Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y 
      de Estudios Avanzados del Instituto Politecnico Nacional, Irapuato, Guanajuato, 
      Mexico.
FAU - Pena-Cabriales, Juan Jose
AU  - Pena-Cabriales JJ
AD  - Departamento de Biotecnologia y Bioquimica, Unidad Irapuato, Centro de 
      Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, 
      Irapuato, Guanajuato, Mexico.
LA  - eng
SI  - GENBANK/KF318744
SI  - GENBANK/KF318745
SI  - GENBANK/KF318746
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150306
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Nitrogen Isotopes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.18.6.1 (Nitrogenase)
RN  - N762921K75 (Nitrogen)
RN  - OC7TV75O83 (Acetylene)
SB  - IM
MH  - Acetylene/metabolism
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacillus/drug effects/*physiology
MH  - Electrophoresis, Agar Gel
MH  - In Situ Hybridization, Fluorescence
MH  - Intracellular Space/*microbiology
MH  - Molecular Sequence Data
MH  - Nitrogen/pharmacology
MH  - *Nitrogen Fixation
MH  - Nitrogen Isotopes
MH  - Nitrogenase/metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Symbiosis/drug effects
MH  - Ustilago/drug effects/growth & development/*physiology/ultrastructure
OTO - NOTNLM
OT  - Bacillus pumilus
OT  - Ustilago maydis
OT  - endosymbiosis
OT  - nifH gene
OT  - nitrogen fixation
OT  - nitrogenase
EDAT- 2015/03/11 06:00
MHDA- 2016/04/27 06:00
CRDT- 2015/03/11 06:00
PHST- 2014/09/26 00:00 [received]
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
AID - 10.1111/nph.13359 [doi]
PST - ppublish
SO  - New Phytol. 2015 Aug;207(3):769-77. doi: 10.1111/nph.13359. Epub 2015 Mar 6.