PMID- 25756408
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150624
LR  - 20150423
IS  - 1539-2465 (Electronic)
IS  - 1536-0652 (Linking)
VI  - 28
IP  - 4
DP  - 2015 May
TI  - Gene expression profiling analysis of patients with ankylosing spondylitis.
PG  - E244-50
LID - 10.1097/BSD.0000000000000266 [doi]
AB  - STUDY DESIGN: A comprehensive bioinformatics analysis of genes which may have 
      correlations with ankylosing spondylitis (AS). OBJECTIVE: To study the mechanisms 
      of AS by analyzing microarray of GSE25101. SUMMARY OF BACKGROUND DATA: AS is an 
      inflammatory arthritis that can lead to chronic pain and disability. MATERIALS 
      AND METHODS: GSE25101 was downloaded from Gene Expression Omnibus including 16 AS 
      patients and 16 normal controls. The differentially expressed genes (DEGs) were 
      screened using limma package in Bioconductor and miRNAs targeted DEGs were 
      predicted. Then, gene ontology and pathway enrichment analysis of DEGs was 
      performed using Database for Annotation, Visualization, and Integrated Discovery. 
      Besides, the interaction relationships of the proteins encoded by DEGs were 
      searched by STRING, and the protein-protein interaction (PPI) network was 
      visualized by Cytoscape. Moreover, modules analysis of PPI network was performed 
      using Clique Percolation Method in CFinder. RESULTS: A total of 284 DEGs were 
      screened and 1899 miRNA-mRNA regulatory pairs were obtained. Both myosin heavy 
      chain 9 (MYH9) and B-cell chronic lymphocytic leukemia/lymphoma 11B (BCL11B) were 
      targeted by has-miR-124 and has-miR-363. Function enrichment analyze indicated 
      that these DEGs, especially, downregulated protein tyrosine phosphatase 
      receptor-type C (PTPRC), cluster of differentiation 3gamma (CD3G), cluster of 
      differentiation 247 (CD247), cluster of differentiation 4 (CD4), interleukin 7 
      receptor (IL7R), MYH9, and BCL11B were associated with regulation of immune 
      response. Meanwhile, CD4 (degree=25), perforin 1 (PRF1) (degree=15), PTPRC 
      (degree=13), and CD247 (degree=9) had higher connectivity degrees in the PPI 
      network for the DEGs. And they might be involved in AS by interacting with other 
      genes in module B (eg, PTPRC-IL7R, CD3G-CD247, and PRF1-PTPRC). CONCLUSION: MYH9, 
      BCL11B, PTPRC, CD3G, CD247, CD4, IL7R, and PRF1 might have a correlation with AS.
FAU - Shi, Zhicai
AU  - Shi Z
AD  - Department of Orthopedics, Changhai Hospital of Shanghai, Shanghai, China.
FAU - Li, Quan
AU  - Li Q
FAU - Cai, Bin
AU  - Cai B
FAU - Ran, Bo
AU  - Ran B
FAU - Li, Ming
AU  - Li M
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - United States
TA  - J Spinal Disord Tech
JT  - Journal of spinal disorders & techniques
JID - 101140323
RIN - J Spinal Disord Tech. 2015 May;28(4):E250. PMID: 25901795
EDAT- 2015/03/11 06:00
MHDA- 2015/03/11 06:01
CRDT- 2015/03/11 06:00
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2015/03/11 06:01 [medline]
AID - 10.1097/BSD.0000000000000266 [doi]
PST - ppublish
SO  - J Spinal Disord Tech. 2015 May;28(4):E244-50. doi: 10.1097/BSD.0000000000000266.