PMID- 25756516
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20220309
IS  - 1555-8576 (Electronic)
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 16
IP  - 2
DP  - 2015
TI  - Calcium calmodulin dependent kinase kinase 2 - a novel therapeutic target for 
      gastric adenocarcinoma.
PG  - 336-45
LID - 10.4161/15384047.2014.972264 [doi]
AB  - Gastric cancer is one of the most common gastrointestinal malignancies and is 
      associated with poor prognosis. Exploring alterations in the proteomic landscape 
      of gastric cancer is likely to provide potential biomarkers for early detection 
      and molecules for targeted therapeutic intervention. Using iTRAQ-based 
      quantitative proteomic analysis, we identified 22 proteins that were 
      overexpressed and 17 proteins that were downregulated in gastric tumor tissues as 
      compared to the adjacent normal tissue. Calcium/calmodulin-dependent protein 
      kinase kinase 2 (CAMKK2) was found to be 7-fold overexpressed in gastric tumor 
      tissues. Immunohistochemical labeling of tumor tissue microarrays for validation 
      of CAMKK2 overexpression revealed that it was indeed overexpressed in 94% (92 of 
      98) of gastric cancer cases. Silencing of CAMKK2 using siRNA significantly 
      reduced cell proliferation, colony formation and invasion of gastric cancer 
      cells. Our results demonstrate that CAMKK2 signals in gastric cancer through AMPK 
      activation and suggest that CAMKK2 could be a novel therapeutic target in gastric 
      cancer.
FAU - Subbannayya, Yashwanth
AU  - Subbannayya Y
AD  - a Institute of Bioinformatics ; International Technology Park ; Bangalore , 
      India.
FAU - Syed, Nazia
AU  - Syed N
FAU - Barbhuiya, Mustafa A
AU  - Barbhuiya MA
FAU - Raja, Remya
AU  - Raja R
FAU - Marimuthu, Arivusudar
AU  - Marimuthu A
FAU - Sahasrabuddhe, Nandini
AU  - Sahasrabuddhe N
FAU - Pinto, Sneha M
AU  - Pinto SM
FAU - Manda, Srikanth Srinivas
AU  - Manda SS
FAU - Renuse, Santosh
AU  - Renuse S
FAU - Manju, H C
AU  - Manju HC
FAU - Zameer, Mohammed Abdul Lateef
AU  - Zameer MA
FAU - Sharma, Jyoti
AU  - Sharma J
FAU - Brait, Mariana
AU  - Brait M
FAU - Srikumar, Kotteazeth
AU  - Srikumar K
FAU - Roa, Juan Carlos
AU  - Roa JC
FAU - Vijaya Kumar, M
AU  - Vijaya Kumar M
FAU - Kumar, K V Veerendra
AU  - Kumar KV
FAU - Prasad, T S Keshava
AU  - Prasad TS
FAU - Ramaswamy, Girija
AU  - Ramaswamy G
FAU - Kumar, Rekha Vijay
AU  - Kumar RV
FAU - Pandey, Akhilesh
AU  - Pandey A
FAU - Gowda, Harsha
AU  - Gowda H
FAU - Chatterjee, Aditi
AU  - Chatterjee A
LA  - eng
GR  - HHSN268201000032C/PHS HHS/United States
GR  - U24CA160036/CA/NCI NIH HHS/United States
GR  - U54GM103520/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Proteome)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Kinase)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Adenocarcinoma/drug therapy/*metabolism
MH  - Antineoplastic Agents/*pharmacology/therapeutic use
MH  - Calcium-Calmodulin-Dependent Protein Kinase Kinase/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Enzyme Activation
MH  - Gene Expression
MH  - Gene Silencing
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Targeted Therapy
MH  - Protein Kinase Inhibitors/*pharmacology/therapeutic use
MH  - Proteome
MH  - Proteomics
MH  - Reproducibility of Results
MH  - Stomach Neoplasms/drug therapy/*metabolism
PMC - PMC4622549
OTO - NOTNLM
OT  - LC-MS/MS, Liquid chromatography-tandem mass spectrometry
OT  - PSM, Peptide spectrum match
OT  - SCX, Strong cation exchange chromatography
OT  - iTRAQ, Isobaric tags for relative and absolute quantitation
OT  - in vitro labeling
OT  - invasion
OT  - mass spectrometry
OT  - proliferation
OT  - siRNA, short interfering RNA
OT  - targeted therapy
EDAT- 2015/03/11 06:00
MHDA- 2015/12/15 06:00
PMCR- 2016/03/10
CRDT- 2015/03/11 06:00
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2016/03/10 00:00 [pmc-release]
AID - 972264 [pii]
AID - 10.4161/15384047.2014.972264 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2015;16(2):336-45. doi: 10.4161/15384047.2014.972264.