PMID- 25757971
OWN - NLM
STAT- MEDLINE
DCOM- 20160616
LR  - 20181202
IS  - 2095-4352 (Print)
VI  - 27
IP  - 3
DP  - 2015 Mar
TI  - [Effect of anisodamine on myocardial connexin 43 expression in pig after 
      resuscitation from cardiac arrest].
PG  - 209-12
LID - 10.3760/cma.j.issn.2095-4352.2015.03.010 [doi]
AB  - OBJECTIVE: To investigate the effect of anisodamine on the expression of connexin 
      43 (Cx43) in swine ventricular myocardium after resuscitation from cardiac 
      arrest. METHODS: The experiment was conducted on healthy pigs, and they were 
      randomly divided into three groups, namely sham group, epinephrine group (control 
      group) and anisodamine group (experimental group, animals were resuscitated 
      combined with injection of 0.4 mg/kg of anisodamine), with 5 pigs in each group. 
      Model of ventricular fibrillation was reproduced by alternating current 
      challenge, and cardiopulmonary resuscitation (CPR) was performed 8 minutes after 
      cardiac arrest. Left ventricular myocardium was harvested at 24 hours after 
      restoration of spontaneous circulation. The expression and distribution of Cx43 
      were observed by immunofluorescence, Cx43 mRNA expression was assessed with 
      reverse transcription-polymerase chain reaction (RT-PCR), and the protein 
      expressions of Cx43 and phosphorylation of Cx43 (p-Cx43) were analyzed by Western 
      Blot. RESULTS: The positive expression of Cx43 in ventricular muscle was 
      distributed uniformly, mostly at the end-to-end linkage of myocardial cells, with 
      a few side-to-side linkage in sham group. The positive expression of Cx43 in 
      control group was significantly weaker than that in the sham group, and the 
      signal intensity was significantly declined (4.35+/-2.10 vs. 10.02+/-3.66, P < 0.01). 
      The positive expression of Cx43 at the end-to-end linkage and side-to-side 
      linkage was irregular in experimental group, and the signal intensity was 
      obviously higher than that in the control group (7.91+/-2.54 vs. 4.35+/-2.10, P < 
      0.05), but it was significantly weaker than that in the sham group (7.91+/-2.54 vs. 
      10.02+/-3.66, P < 0.05). For control group and experimental group, the Cx43 mRNA 
      and protein expressions were significantly lower than those of the sham group 
      [Cx43 mRNA (A value): 0.32+/-0.05, 0.32+/-0.03 vs. 0.48+/-0.07; Cx43 protein (A value): 
      0.43+/-0.03, 0.50+/-0.07 vs. 0.65+/-0.04, all P < 0.01], and there were no significant 
      differences between experimental group and control group (all P > 0.05). The 
      p-Cx43 protein expression of control group was significantly lower than that of 
      the sham group (A value: 0.22+/-0.03 vs. 0.37+/-0.06, P < 0.01), and it was 
      significantly higher in the experiment group than that in the control group (A 
      value: 0.29+/-0.07 vs. 0.22+/-0.03, P < 0.01), but there was no significant 
      difference with the sham group (P > 0.05). No significant difference in 
      p-Cx43/Cx43 was found among sham, control, and experiment groups (0.57+/-0.09, 
      0.51+/-0.05, 0.58+/-0.06, all P > 0.05). CONCLUSIONS: Anisodamine can improve the 
      abnormal expression of Cx43 in ventricular muscle of pigs with cardiac arrest, 
      which may be related to the protection effect of anisodamine on cardiac 
      conduction.
FAU - Liu, Yahua
AU  - Liu Y
AD  - Department of Emergency, General Hospital of Chinese People Armed Police Forces, 
      Beijing 100039, China. Corresponding author: Shen Hong, Department of Emergency, 
      General Hospital of PLA, Beijing 100853, China, Email: shenhong@em120.com.
FAU - Shen, Hong
AU  - Shen H
FAU - Wang, Lixiang
AU  - Wang L
FAU - Yang, Huining
AU  - Yang H
FAU - Ma, Lizhi
AU  - Ma L
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (Connexin 43)
RN  - 0 (Solanaceous Alkaloids)
RN  - 01343Q8EL8 (anisodamine)
SB  - IM
MH  - Animals
MH  - Cardiopulmonary Resuscitation
MH  - Connexin 43
MH  - *Heart Arrest
MH  - Myocardium
MH  - Phosphorylation
MH  - Solanaceous Alkaloids
MH  - Swine
MH  - Ventricular Fibrillation
EDAT- 2015/03/12 06:00
MHDA- 2016/06/17 06:00
CRDT- 2015/03/12 06:00
PHST- 2015/03/12 06:00 [entrez]
PHST- 2015/03/12 06:00 [pubmed]
PHST- 2016/06/17 06:00 [medline]
AID - 10.3760/cma.j.issn.2095-4352.2015.03.010 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2015 Mar;27(3):209-12. doi: 
      10.3760/cma.j.issn.2095-4352.2015.03.010.