PMID- 25758848
OWN - NLM
STAT- MEDLINE
DCOM- 20160128
LR  - 20150429
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 44
IP  - 2
DP  - 2015 Jun
TI  - Dietary Aloe vera supplementation on growth performance, some haemato-biochemical 
      parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).
PG  - 504-14
LID - S1050-4648(15)00101-1 [pii]
LID - 10.1016/j.fsi.2015.03.002 [doi]
AB  - This study investigated effects of dietary Aloe vera on growth performance, some 
      haemato-biochemical parameters and disease resistance against Streptococcus iniae 
      in tilapia (GIFT). Five groups were designed including a basal diet (control) and 
      100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, 
      which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera 
      supplemented diet significantly improved (p < 0.05) weight gain, absolute growth 
      rate and specific growth rate. Feed intake significantly increased in fish fed 
      with A. vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion 
      ratio, and hepatosomatic index were significantly enhanced in 4% A. vera 
      supplemented fish over unsupplemented ones (p < 0.05). Several 
      haemato-biochemical indices were examined before and after fish were challenged 
      with S. iniae pathogen containing 7.7 x 10(6) CFU cells mL(-1). A. vera 
      supplemented fish showed a significant increase (p < 0.05) in red blood cells, 
      hematocrits (Hb), hemoglobin (Hb), white blood cells (WBC), neutrophils, 
      monocytes, eosinophils, serum total protein, glucose and cortisol after challenge 
      when compared to unsupplemented ones. Meanwhile, 4% A. vera supplemented fish 
      showed a decrease (p < 0.05) in RBC, Hb, Ht, WBC, and mean corpuscular hemoglobin 
      (MCH) after challenge compared to unsupplemented ones and other supplemented 
      ones. In addition, lower mean corpuscular volume values (MCV) (p < 0.05) were 
      observed in fish fed with A. vera diet at 2% and 4% A. vera/kg feed than those 
      fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed 
      significantly higher values (p < 0.05) of mean corpuscular hemoglobin 
      concentration (MCHC) than those fed unsupplemented diet and 4% A. vera 
      supplemented diet. There was a significant increase (p < 0.05) in the neutrophil 
      to lymphocyte ratio (N/L) within experimental groups after challenge; N/L ratio 
      in A. vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg 
      feed increased significantly (p < 0.05) throughout challenge period; while those 
      fed 4% A. vera supplemented diet maintained higher values at all experimental 
      stages among groups. There was a significant correlation (p < 0.05, r = 0.53) 
      between N/L ratio and glucose concentration, 96 h after challenge. Aloe had no 
      significant effect (p > 0.05) on the survival of the fish when compared to the 
      control; no mortality was recorded in challenge trial. Overall, our results 
      indicated that dietary aloe supplementation could improve growth, feed 
      utilization, and haemato-biochemical parameters of cultured tilapia.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Gabriel, Ndakalimwe Naftal
AU  - Gabriel NN
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 
      Directorate of Aquaculture, Ministry of Fisheries and Marine Resources, Hardap, 
      Namibia.
FAU - Qiang, Jun
AU  - Qiang J
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 
      China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China.
FAU - He, Jie
AU  - He J
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Ma, Xin Yu
AU  - Ma XY
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Kpundeh, Mathew D
AU  - Kpundeh MD
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Xu, Pao
AU  - Xu P
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 
      China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China. Electronic address: xup@ffr.cn.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20150307
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Blood Glucose)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Aloe/*chemistry
MH  - Animals
MH  - Blood Glucose
MH  - *Dietary Supplements
MH  - Disease Resistance/*drug effects/immunology
MH  - Dose-Response Relationship, Drug
MH  - Eating/drug effects
MH  - Lymphocytes/drug effects
MH  - Neutrophils/drug effects
MH  - Plant Extracts/analysis/*pharmacology
MH  - Streptococcus/*immunology
MH  - Tilapia/*growth & development/*immunology
MH  - Weight Gain/drug effects
OTO - NOTNLM
OT  - Bacterium
OT  - Challenge
OT  - Cichlidae
OT  - Feeding
OT  - Herbs
OT  - Immunity
OT  - Oreochromis
OT  - Survival
EDAT- 2015/03/12 06:00
MHDA- 2016/01/29 06:00
CRDT- 2015/03/12 06:00
PHST- 2014/12/19 00:00 [received]
PHST- 2015/02/20 00:00 [revised]
PHST- 2015/03/01 00:00 [accepted]
PHST- 2015/03/12 06:00 [entrez]
PHST- 2015/03/12 06:00 [pubmed]
PHST- 2016/01/29 06:00 [medline]
AID - S1050-4648(15)00101-1 [pii]
AID - 10.1016/j.fsi.2015.03.002 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2015 Jun;44(2):504-14. doi: 10.1016/j.fsi.2015.03.002. 
      Epub 2015 Mar 7.