PMID- 25759304
OWN - NLM
STAT- MEDLINE
DCOM- 20160510
LR  - 20150721
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 39
IP  - 8
DP  - 2015 Aug
TI  - Biological characteristics of adipose tissue-derived stem cells labeled with 
      amine-surface-modified superparamagnetic iron oxide nanoparticles.
PG  - 899-909
LID - 10.1002/cbin.10457 [doi]
AB  - Cell labeling and tracking are becoming increasingly important areas within the 
      field of stem cell transplantation. The ability to track the migration and 
      distribution of implanted cells is critical to understanding the beneficial 
      effects and mechanisms of stem cell therapy. The present study investigated the 
      effects of amine-surface-modified superparamagnetic iron oxide (SPIO) 
      nanoparticles on the biological properties of human adipose tissue-derived stem 
      cells (hADSCs). Monodisperse hydrophobic magnetite (Fe3 O4 ) nanoparticles were 
      prepared using silicon and surface-modified with amine coating. Cell viability, 
      proliferation, differentiation potential, and surface marker expression were 
      evaluated. The magnetic particles (10-18 nm) displayed high labeling efficiency 
      and stability in hADSCs. SPIO-labeled cells produced a hypointense signal and 
      were effectively visualized by MRI for up to 21 days. The results of MTT 
      proliferation assays and flow cytometry analysis demonstrated that SPIOs were 
      biocompatible, viz. the labeling process did not cause cell death or apoptosis 
      and had no side effects on cell proliferation. In vivo experiments showed that 
      the magnetic particles did not affect liver and kidney function. The successful 
      and stable labeling of hADSCs combined with efficient magnetic tropism 
      demonstrates that SPIOs are promising candidates for hADSC tracking in 
      hADSC-based cell therapy applications.
CI  - (c) 2015 International Federation for Cell Biology.
FAU - Wang, Nan
AU  - Wang N
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
AD  - Central Laboratory, Sixth People's Hospital of Dalian, Dalian 116031, P. R. 
      China.
FAU - Zhao, Jing-Yuan
AU  - Zhao JY
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
FAU - Guan, Xin
AU  - Guan X
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
FAU - Dong, Yue
AU  - Dong Y
AD  - Department of Radiology, First Affiliated Hospital of Dalian Medical University, 
      Dalian 116021, P. R. China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
FAU - Zhou, Xiang
AU  - Zhou X
AD  - Department of Neurology, First Affiliated Hospital of Dalian Medical University, 
      Dalian 116021, P. R. China.
FAU - Wu, Ren'an
AU  - Wu R
AD  - Dalian Institute of Chemical Physics, Dalian 116021, P. R. China.
FAU - Du, Yue
AU  - Du Y
AD  - College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, P. 
      R. China.
FAU - Zhao, Liang
AU  - Zhao L
AD  - Dalian Institute of Chemical Physics, Dalian 116021, P. R. China.
FAU - Zou, Wei
AU  - Zou W
AD  - College of Life Sciences, Liaoning Normal University, Dalian 116011, P. R. China.
FAU - Han, Chao
AU  - Han C
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
FAU - Song, Lin
AU  - Song L
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
AD  - School of Life Science and Biotechnology, Dalian University of Technology, Dalian 
      116024, P. R. China.
FAU - Sun, Bo
AU  - Sun B
AD  - Department of Neurology, First Affiliated Hospital of Dalian Medical University, 
      Dalian 116021, P. R. China.
FAU - Liu, Yan
AU  - Liu Y
AD  - Department of Clinical Laboratory, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
FAU - Liu, Jing
AU  - Liu J
AD  - Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116021, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150413
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Amines)
RN  - 0 (Ferric Compounds)
RN  - 0 (Magnetite Nanoparticles)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Adipose Tissue/*cytology/metabolism
MH  - Amines/chemistry
MH  - Cell Differentiation/physiology
MH  - Ferric Compounds/chemistry
MH  - Humans
MH  - Magnetite Nanoparticles/*chemistry
MH  - Primary Cell Culture
MH  - Stem Cell Transplantation/methods
MH  - Stem Cells/*cytology/metabolism
OTO - NOTNLM
OT  - MRI
OT  - human adipose tissue-derived stem cells
OT  - superparamagnetic iron oxide nanoparticles
OT  - transplant
EDAT- 2015/03/12 06:00
MHDA- 2016/05/11 06:00
CRDT- 2015/03/12 06:00
PHST- 2014/07/17 00:00 [received]
PHST- 2015/02/25 00:00 [accepted]
PHST- 2015/03/12 06:00 [entrez]
PHST- 2015/03/12 06:00 [pubmed]
PHST- 2016/05/11 06:00 [medline]
AID - 10.1002/cbin.10457 [doi]
PST - ppublish
SO  - Cell Biol Int. 2015 Aug;39(8):899-909. doi: 10.1002/cbin.10457. Epub 2015 Apr 13.