PMID- 25759837
OWN - NLM
STAT- MEDLINE
DCOM- 20151201
LR  - 20181113
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2015
DP  - 2015
TI  - Characterization of Toll-like receptor-4 (TLR-4) in the spleen and thymus of 
      Swiss albino mice and its modulation in experimental endotoxemia.
PG  - 137981
LID - 10.1155/2015/137981 [doi]
LID - 137981
AB  - Expression of innate immune receptors varies among organs and species and within 
      different strains among the same species; thus, periodic classification of 
      different pattern recognition receptors in the available strains is necessary to 
      initiate different therapeutic approaches to combat inflammation. On 
      characterization of TLR-4 in spleen and thymus of Swiss albino mice--with no 
      reports of TLR-4 expression--induced with endotoxemia, it was found that the mode 
      of expression varied among the organs at both mRNA and protein level in a 
      time-dependent manner. Their functionality was verified by measuring 
      proinflammatory and anti-inflammatory cytokines. In the in vitro study using 
      isolated macrophages and lymphocytes from the same organs, the expression of 
      TLR-4 after a shorter period of LPS stimulation was verified. The results 
      substantiated the potent role of macrophage on LPS challenge compared to 
      lymphocytes. The diverse pattern of TLR-4 expression on different cell population 
      indicated their distinct functional activity in LPS-endotoxemia. It may be 
      hypothesized that the expression patterns of TLR-4 could be different based on 
      the anatomical localization and the varying bacterial milieu or bacterial 
      endotoxin encountered in each anatomical location. Thus, blocking TLR-4 or 
      administering IL-6 or IL-10 might impart protection against endotoxemia in the 
      clinical field.
FAU - Ghosh, Chandrayee
AU  - Ghosh C
AD  - Department of Physiology, Immunology Laboratory, University of Calcutta, 
      University Colleges of Science and Technology, 92 APC Road, Kolkata, West Bengal 
      700009, India.
FAU - Bishayi, Biswadev
AU  - Bishayi B
AD  - Department of Physiology, Immunology Laboratory, University of Calcutta, 
      University Colleges of Science and Technology, 92 APC Road, Kolkata, West Bengal 
      700009, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150205
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 4)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Animals
MH  - C-Reactive Protein
MH  - Culture Media, Conditioned/metabolism
MH  - Cytokines/blood
MH  - Disease Models, Animal
MH  - Endotoxemia/genetics/*immunology/*metabolism/pathology
MH  - Gene Expression
MH  - *Immunomodulation
MH  - Inflammation Mediators/blood
MH  - Lipopolysaccharides/immunology
MH  - Lymphocytes/immunology/metabolism
MH  - Macrophages/immunology/metabolism
MH  - Male
MH  - Mice
MH  - RNA, Messenger/genetics/metabolism
MH  - Spleen/*immunology/*metabolism/pathology
MH  - Thymus Gland/*immunology/*metabolism/pathology
MH  - Toll-Like Receptor 4/genetics/*metabolism
PMC - PMC4352500
EDAT- 2015/03/12 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/02/05
CRDT- 2015/03/12 06:00
PHST- 2014/10/17 00:00 [received]
PHST- 2015/01/19 00:00 [revised]
PHST- 2015/01/19 00:00 [accepted]
PHST- 2015/03/12 06:00 [entrez]
PHST- 2015/03/12 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/02/05 00:00 [pmc-release]
AID - 10.1155/2015/137981 [doi]
PST - ppublish
SO  - J Immunol Res. 2015;2015:137981. doi: 10.1155/2015/137981. Epub 2015 Feb 5.