PMID- 25759941
OWN - NLM
STAT- MEDLINE
DCOM- 20151009
LR  - 20150407
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 7
DP  - 2015 Apr 7
TI  - Attractive design: an elution solvent optimization platform for 
      magnetic-bead-based fractionation using digital microfluidics and design of 
      experiments.
PG  - 3902-10
LID - 10.1021/ac504697r [doi]
AB  - There is great interest in the development of integrated tools allowing for 
      miniaturized sample processing, including solid phase extraction (SPE). We 
      introduce a new format for microfluidic SPE relying on C18-functionalized 
      magnetic beads that can be manipulated in droplets in a digital microfluidic 
      platform. This format provides the opportunity to tune the amount (and 
      potentially the type) of stationary phase on-the-fly, and allows the removal of 
      beads after the extraction (to enable other operations in same device-space), 
      maintaining device reconfigurability. Using the new method, we employed a design 
      of experiments (DOE) operation to enable automated on-chip optimization of 
      elution solvent composition for reversed phase SPE of a model system. Further, 
      conditions were selected to enable on-chip fractionation of multiple analytes. 
      Finally, the method was demonstrated to be useful for online cleanup of extracts 
      from dried blood spot (DBS) samples. We anticipate this combination of features 
      will prove useful for separating a wide range of analytes, from small molecules 
      to peptides, from complex matrices.
FAU - Lafreniere, Nelson M
AU  - Lafreniere NM
AD  - daggerDepartment of Chemistry, University of Toronto, 80 St George Street, Toronto, 
      Ontario M5S 3H6, Canada.
FAU - Mudrik, Jared M
AU  - Mudrik JM
AD  - daggerDepartment of Chemistry, University of Toronto, 80 St George Street, Toronto, 
      Ontario M5S 3H6, Canada.
FAU - Ng, Alphonsus H C
AU  - Ng AH
AD  - double daggerInstitute of Biomaterials and Biomedical Engineering, 164 College Street, 
      Toronto, Ontario M5S 3G9, Canada.
FAU - Seale, Brendon
AU  - Seale B
AD  - daggerDepartment of Chemistry, University of Toronto, 80 St George Street, Toronto, 
      Ontario M5S 3H6, Canada.
FAU - Spooner, Neil
AU  - Spooner N
AD  -  section signPlatform Technologies and Science Drug Metabolism and Pharmacokinetics, 
      GlaxoSmithKline Research and Development, Ware, Hertfordshire SG12 0DP, United 
      Kingdom.
FAU - Wheeler, Aaron R
AU  - Wheeler AR
AD  - daggerDepartment of Chemistry, University of Toronto, 80 St George Street, Toronto, 
      Ontario M5S 3H6, Canada.
AD  - double daggerInstitute of Biomaterials and Biomedical Engineering, 164 College Street, 
      Toronto, Ontario M5S 3G9, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150311
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aminoquinolines)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Solvents)
RN  - 9041-90-1 (Angiotensin I)
RN  - U34EAV21TG (8-aminoquinoline)
SB  - IM
MH  - Aminoquinolines/*analysis
MH  - Angiotensin I/*analysis
MH  - *Dried Blood Spot Testing
MH  - Equipment Design
MH  - Humans
MH  - Magnetite Nanoparticles/*chemistry
MH  - *Microfluidic Analytical Techniques/instrumentation/methods
MH  - Solid Phase Extraction
MH  - Solvents/*chemistry
EDAT- 2015/03/12 06:00
MHDA- 2015/10/10 06:00
CRDT- 2015/03/12 06:00
PHST- 2015/03/12 06:00 [entrez]
PHST- 2015/03/12 06:00 [pubmed]
PHST- 2015/10/10 06:00 [medline]
AID - 10.1021/ac504697r [doi]
PST - ppublish
SO  - Anal Chem. 2015 Apr 7;87(7):3902-10. doi: 10.1021/ac504697r. Epub 2015 Mar 11.