PMID- 25760926
OWN - NLM
STAT- MEDLINE
DCOM- 20151217
LR  - 20220321
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 35
IP  - 5
DP  - 2015 May
TI  - miR-218 inhibits the invasion and migration of colon cancer cells by targeting 
      the PI3K/Akt/mTOR signaling pathway.
PG  - 1301-8
LID - 10.3892/ijmm.2015.2126 [doi]
AB  - Colon cancer is one of the most common and lethal malignancies worldwide. Despite 
      major advances in the treatment of colon cancer, the prognosis remains very poor. 
      Thus, novel and effective therapies for colon cancer are urgently needed. In the 
      present study, the expression status of miR-218 and the role of the 
      phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of 
      rapamycin (mTOR) pathway were investigated in colon cancer samples. Firstly, we 
      observed that miR-218 expression was significantly reduced, while PI3K/Akt/mTOR 
      pathway activity was enhanced. The overexpression of miR-218 suppressed the 
      proliferation, migration and invasion of LoVo colon cancer cells, whereas the 
      inhibition of miR-218 promoted these processes. Furthermore, the PI3K/Akt/mTOR 
      signaling pathway was identified as a direct target of miR-218. The upregulation 
      of miR-218 inhibited the activation of the PI3K/Akt/mTOR signaling pathway, as 
      well as the expression of matrix metalloproteinase (MMP)9. The downregulation of 
      miR-218 activated the PI3K/Akt/mTOR signaling pathway and promoted MMP9 
      expression. Taken together, our results demonstrate that miR-218 suppresses the 
      proliferation, migration and invasion of LoVo colon cancer cells by targeting the 
      PI3K/Akt/mTOR signaling pathway and MMP9. Our data indicate that miR-218 is a 
      potential target in the treatment of colon cancer.
FAU - Zhang, Xiangliang
AU  - Zhang X
AD  - Department of Abdominal Surgery (Section 2), The Affiliated Cancer Hospital of 
      Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Shi, Huijuan
AU  - Shi H
AD  - Department of Pathology, The First Affiliated Hospital of Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Tang, Hongsheng
AU  - Tang H
AD  - Department of Abdominal Surgery (Section 2), The Affiliated Cancer Hospital of 
      Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Fang, Zhiyuan
AU  - Fang Z
AD  - Department of Abdominal Surgery (Section 2), The Affiliated Cancer Hospital of 
      Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Wang, Jiping
AU  - Wang J
AD  - Department of Surgery, Brigham and Women's Hospital affiliated to Harvard Medical 
      School, Boston, MA 02115, USA.
FAU - Cui, Shuzhong
AU  - Cui S
AD  - Department of Abdominal Surgery (Section 2), The Affiliated Cancer Hospital of 
      Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150306
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (MIRN218 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation
MH  - Colonic Neoplasms/*genetics/*metabolism/pathology
MH  - Gene Expression
MH  - Humans
MH  - Matrix Metalloproteinase 9/genetics
MH  - MicroRNAs/*genetics
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - RNA Interference
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2015/03/12 06:00
MHDA- 2015/12/19 06:00
CRDT- 2015/03/12 06:00
PHST- 2014/12/12 00:00 [received]
PHST- 2015/02/26 00:00 [accepted]
PHST- 2015/03/12 06:00 [entrez]
PHST- 2015/03/12 06:00 [pubmed]
PHST- 2015/12/19 06:00 [medline]
AID - 10.3892/ijmm.2015.2126 [doi]
PST - ppublish
SO  - Int J Mol Med. 2015 May;35(5):1301-8. doi: 10.3892/ijmm.2015.2126. Epub 2015 Mar 
      6.