PMID- 25764225
OWN - NLM
STAT- MEDLINE
DCOM- 20160308
LR  - 20211203
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 15
IP  - 12
DP  - 2015 Jun
TI  - Phosphoproteome characterization reveals that Sendai virus infection activates 
      mTOR signaling in human epithelial cells.
PG  - 2087-97
LID - 10.1002/pmic.201400586 [doi]
AB  - Sendai virus (SeV) is a common respiratory pathogen in mice, rats, and hamsters. 
      Host cell recognition of SeV is mediated by pathogen recognition receptors, which 
      recognize viral components and induce intracellular signal transduction pathways 
      that activate the antiviral innate immune response. Viruses use host proteins to 
      control the activities of signaling proteins and their downstream targets, and 
      one of the most important host protein modifications regulated by viral infection 
      is phosphorylation. In this study, we used phosphoproteomics combined with 
      bioinformatics to get a global view of the signaling pathways activated during 
      SeV infection in human lung epithelial cells. We identified altogether 1347 
      phosphoproteins, and our data shows that SeV infection induces major changes in 
      protein phosphorylation affecting the phosphorylation of almost one thousand host 
      proteins. Bioinformatics analysis showed that SeV infection activates known 
      pathways including MAPK signaling, as well as signaling pathways previously not 
      linked to SeV infection including Rho family of GTPases, HIPPO signaling, and 
      mammalian target of rapamycin (mTOR)-signaling pathway. Further, we performed 
      functional studies with mTOR inhibitors and siRNA approach, which revealed that 
      mTOR signaling is needed for both the host IFN response as well as viral protein 
      synthesis in SeV-infected human lung epithelial cells.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Ohman, Tiina
AU  - Ohman T
AD  - Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
FAU - Soderholm, Sandra
AU  - Soderholm S
AD  - Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
AD  - Finnish Institute of Occupational Health, Helsinki, Finland.
FAU - Paidikondala, Maruthibabu
AU  - Paidikondala M
AD  - Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
FAU - Lietzen, Niina
AU  - Lietzen N
AD  - Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
FAU - Matikainen, Sampsa
AU  - Matikainen S
AD  - Finnish Institute of Occupational Health, Helsinki, Finland.
FAU - Nyman, Tuula A
AU  - Nyman TA
AD  - Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150427
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 9008-11-1 (Interferons)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Computational Biology
MH  - Cricetinae
MH  - Epithelial Cells/cytology/*metabolism
MH  - Humans
MH  - Interferons/metabolism
MH  - Lung Neoplasms/*metabolism/pathology/virology
MH  - Mice
MH  - Phosphoproteins/genetics/*metabolism
MH  - Phosphorylation
MH  - Protein Array Analysis
MH  - Proteomics/*methods
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Real-Time Polymerase Chain Reaction
MH  - Respirovirus Infections/*metabolism/virology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sendai virus/*physiology
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - Bioinformatics
OT  - IFN response
OT  - Phosphoproteome
OT  - Virus infection
OT  - mTOR signaling
EDAT- 2015/03/13 06:00
MHDA- 2016/03/10 06:00
CRDT- 2015/03/13 06:00
PHST- 2014/12/10 00:00 [received]
PHST- 2015/02/24 00:00 [revised]
PHST- 2015/03/06 00:00 [accepted]
PHST- 2015/03/13 06:00 [entrez]
PHST- 2015/03/13 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
AID - 10.1002/pmic.201400586 [doi]
PST - ppublish
SO  - Proteomics. 2015 Jun;15(12):2087-97. doi: 10.1002/pmic.201400586. Epub 2015 Apr 
      27.