PMID- 25769013 OWN - NLM STAT- MEDLINE DCOM- 20151130 LR - 20181113 IS - 1741-2552 (Electronic) IS - 1741-2560 (Print) IS - 1741-2552 (Linking) VI - 12 IP - 2 DP - 2015 Apr TI - Differential intensity-dependent effects of magnetic stimulation on the longest neurites and shorter dendrites in neuroscreen-1 cells. PG - 026013 LID - 10.1088/1741-2560/12/2/026013 [doi] AB - OBJECTIVE: Magnetic stimulation (MS) is a potential treatment for neuropsychiatric disorders. This study investigates whether MS-regulated neuronal activity can translate to specific changes in neuronal arborization and thus regulate synaptic activity and function. APPROACH: To test our hypotheses, we examined the effects of MS on neurite growth of neuroscreen-1 (NS-1) cells over the pulse frequencies of 1, 5 and 10 Hz at field intensities controlled via machine output (MO). Cells were treated with either 30% or 40% MO. Due to the nature of circular MS coils, the center region of the gridded coverslip (zone 1) received minimal ( approximately 5%) electromagnetic current density while the remaining area (zone 2) received maximal ( approximately 95%) current density. Plated NS-1 cells were exposed to MS twice per day for three days and then evaluated for length and number of neurites and expression of brain-derived neurotrophic factor (BDNF). MAIN RESULTS: We show that MS dramatically affects the growth of the longest neurites (axon-like) but does not significantly affect the growth of shorter neurites (dendrite-like). Also, MS-induced changes in the longest neurite growth were most evident in zone 1, but not in zone 2. MS effects were intensity-dependent and were most evident in bolstering longest neurite outgrowth, best seen in the 10 Hz MS group. Furthermore, we found that MS-increased BDNF expression and secretion was also frequency-dependent. Taken together, our results show that MS exerts distinct effects when different frequencies and intensities are applied to the neuritic compartments (longest neurite versus shorter dendrite(s)) of NS-1 cells. SIGNIFICANCE: These findings support the concept that MS increases BDNF expression and signaling, which sculpts longest neurite arborization and connectivity by which neuronal activity is regulated. Understanding the mechanisms underlying MS is crucial for efficiently incorporating its use into potential therapeutic strategies. FAU - Lin, Ching-Yi AU - Lin CY AD - Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA. Department of Physical Medicine and Rehabilitation, Cleveland Clinic, Cleveland, OH, USA. FAU - Huang, Whitney J AU - Huang WJ FAU - Li, Kevin AU - Li K FAU - Swanson, Roy AU - Swanson R FAU - Cheung, Brian AU - Cheung B FAU - Lin, Vernon W AU - Lin VW FAU - Lee, Yu-Shang AU - Lee YS LA - eng GR - R01 NS069765/NS/NINDS NIH HHS/United States GR - NS069765/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20150313 PL - England TA - J Neural Eng JT - Journal of neural engineering JID - 101217933 SB - IM MH - Animals MH - Cell Line MH - Cell Size/radiation effects MH - Dendrites/*physiology/radiation effects/ultrastructure MH - Dose-Response Relationship, Radiation MH - Electric Stimulation/*methods MH - *Magnetic Fields MH - Neurites/physiology/radiation effects/ultrastructure MH - Radiation Dosage MH - Rats PMC - PMC4400261 MID - NIHMS673698 EDAT- 2015/03/15 06:00 MHDA- 2015/12/15 06:00 PMCR- 2016/04/01 CRDT- 2015/03/14 06:00 PHST- 2015/03/14 06:00 [entrez] PHST- 2015/03/15 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] PHST- 2016/04/01 00:00 [pmc-release] AID - 10.1088/1741-2560/12/2/026013 [doi] PST - ppublish SO - J Neural Eng. 2015 Apr;12(2):026013. doi: 10.1088/1741-2560/12/2/026013. Epub 2015 Mar 13.