PMID- 25769457
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20201209
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 31
IP  - 8
DP  - 2015 Aug
TI  - Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 
      Infection in CD4(+) T Cells.
PG  - 806-16
LID - 10.1089/AID.2014.0313 [doi]
AB  - HIV-1 interacts with numerous cellular proteins during viral replication. 
      Identifying such host proteins and characterizing their roles in HIV-1 infection 
      can deepen our understanding of the dynamic interplay between host and pathogen. 
      We previously identified non-POU domain-containing octamer-binding protein (NonO 
      or p54nrb) as one of host factors associated with catalytically active 
      preintegration complexes (PIC) of HIV-1 in infected CD4(+) T cells. NonO is 
      involved in nuclear processes including transcriptional regulation and RNA 
      splicing. Although NonO has been identified as an HIV-1 interactant in several 
      recent studies, its role in HIV-1 replication has not been characterized. We 
      investigated the effect of NonO on the HIV-1 life cycle in CD4(+) T cell lines 
      and primary CD4(+) T cells using single-cycle and replication-competent HIV-1 
      infection assays. We observed that short hairpin RNA (shRNA)-mediated stable NonO 
      knockdown in a CD4(+) Jurkat T cell line and primary CD4(+) T cells did not 
      affect cell viability or proliferation, but enhanced HIV-1 infection. The 
      enhancement of HIV-1 infection in Jurkat T cells correlated with increased viral 
      reverse transcription and gene expression. Knockdown of NonO expression in Jurkat 
      T cells modestly enhanced HIV-1 gag mRNA expression and Gag protein synthesis, 
      suggesting that viral gene expression and RNA regulation are the predominantly 
      affected events causing enhanced HIV-1 replication in NonO knockdown (KD) cells. 
      Furthermore, overexpression of NonO in Jurkat T cells reduced HIV-1 single-cycle 
      infection by 41% compared to control cells. Our data suggest that NonO negatively 
      regulates HIV-1 infection in CD4(+) T cells, albeit it has modest effects on 
      early and late stages of the viral life cycle, highlighting the importance of 
      host proteins associated with HIV-1 PIC in regulating viral replication.
FAU - St Gelais, Corine
AU  - St Gelais C
AD  - 1 Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio 
      State University , Columbus, Ohio.
FAU - Roger, Jonathan
AU  - Roger J
AD  - 1 Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio 
      State University , Columbus, Ohio.
FAU - Wu, Li
AU  - Wu L
AD  - 1 Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio 
      State University , Columbus, Ohio.
AD  - 2 Department of Microbial Infection and Immunity, The Ohio State University , 
      Columbus, Ohio.
AD  - 3 Comprehensive Cancer Center, The Ohio State University , Columbus, Ohio.
LA  - eng
GR  - R01 AI104483/AI/NIAID NIH HHS/United States
GR  - R21 AI102822/AI/NIAID NIH HHS/United States
GR  - AI102822/AI/NIAID NIH HHS/United States
GR  - AI104483/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150406
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NONO protein, human)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Octamer Transcription Factors)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*immunology/*virology
MH  - Cells, Cultured
MH  - DNA-Binding Proteins
MH  - Gene Knockdown Techniques
MH  - HIV-1/*immunology/*physiology
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Nuclear Matrix-Associated Proteins/antagonists & inhibitors/*metabolism
MH  - Octamer Transcription Factors/antagonists & inhibitors/*metabolism
MH  - RNA-Binding Proteins/antagonists & inhibitors/*metabolism
MH  - *Virus Replication
PMC - PMC4533096
EDAT- 2015/03/15 06:00
MHDA- 2016/04/27 06:00
PMCR- 2016/08/01
CRDT- 2015/03/15 06:00
PHST- 2015/03/15 06:00 [entrez]
PHST- 2015/03/15 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
PHST- 2016/08/01 00:00 [pmc-release]
AID - 10.1089/aid.2014.0313 [pii]
AID - 10.1089/AID.2014.0313 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2015 Aug;31(8):806-16. doi: 10.1089/AID.2014.0313. 
      Epub 2015 Apr 6.