PMID- 25770739
OWN - NLM
STAT- MEDLINE
DCOM- 20150807
LR  - 20150502
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 195
IP  - 2
DP  - 2015 May 15
TI  - Effect of adenosine triphosphate-sensitive potassium activation on peripheral and 
      central pain sensitization.
PG  - 481-7
LID - S0022-4804(15)00064-5 [pii]
LID - 10.1016/j.jss.2015.01.033 [doi]
AB  - BACKGROUND: Alterations in adenosine triphosphate-sensitive potassium (KATP) 
      activity and expression under changing physiological conditions are important 
      adaptive and protective mechanisms. KATP subunit expression is also altered in 
      neuropathic pain; whether these changes are adaptive or deleterious is unclear. 
      We therefore established a skin/muscle incision and retraction (SMIR) rat model 
      of postoperative pain and examined the relationship between pain sensitization 
      and changes in KATP subunit expression. METHODS: Rats were randomly divided into 
      untreated, sham-operation, SMIR, and SMIR + Pinacidil (sulfonylurea receptor 
      [SUR]2-activator) groups. In the SMIR group, skin and muscle were retracted for 1 
      h after incision. In the SMIR + Pinacidil group, Pinacidil was injected 
      intraperitoneally 0.5 h before SMIR or into the spinal myelin sheath 7 d after 
      SMIR. Mechanical withdrawal threshold was used as an index of pain sensitivity. 
      Expression levels and localization of the KATP subunits Kir6.2, Kir6.1, SUR1, and 
      SUR2 were measured by Western blotting and immunofluorescence. RESULTS: A rat 
      postoperative pain model was successfully established, in which SMIR induced 
      mechanical hypersensitivity (allodynia). Notably, significantly increased Kir6.1, 
      Kir6.2, SUR1, and SUR2 protein expression levels were observed in tissues around 
      the incision (P < 0.05). In addition, significantly decreased Kir6.1, SUR2, and 
      SUR1 protein levels were obtained in spinal cord L3-L5. SMIR also starkly 
      increased nerve growth factor expression in the muscle around the incision. 
      Importantly, intrathecal Pinacidil injection inhibited the overexpression of 
      allodynia markers and nerve growth factor. CONCLUSIONS: Hyperexcitability due to 
      spinal Kir6.1 and SUR2 downregulation may be responsible for postoperative pain. 
      SUR2 activation is a potential strategy to inhibit postoperative allodynia.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Shen, Shiren
AU  - Shen S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      China.
FAU - Cao, Su
AU  - Cao S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      China. Electronic address: cao112138353@163.com.
FAU - Huang, Saisai
AU  - Huang S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      China.
FAU - Chen, Junjie
AU  - Chen J
AD  - Department of Anesthesiology, Nantong University, Nantong, China.
LA  - eng
PT  - Journal Article
DEP - 20150128
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Abcc8 protein, rat)
RN  - 0 (Abcc9 protein, rat)
RN  - 0 (KATP Channels)
RN  - 0 (Sulfonylurea Receptors)
SB  - IM
MH  - Animals
MH  - Hyperalgesia/physiopathology
MH  - KATP Channels/*physiology
MH  - Male
MH  - Pain, Postoperative/*physiopathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sulfonylurea Receptors/physiology
OTO - NOTNLM
OT  - K(ATP) subunit
OT  - Pain sensitization
OT  - SMIR
EDAT- 2015/03/17 06:00
MHDA- 2015/08/08 06:00
CRDT- 2015/03/16 06:00
PHST- 2014/05/22 00:00 [received]
PHST- 2015/01/05 00:00 [revised]
PHST- 2015/01/21 00:00 [accepted]
PHST- 2015/03/16 06:00 [entrez]
PHST- 2015/03/17 06:00 [pubmed]
PHST- 2015/08/08 06:00 [medline]
AID - S0022-4804(15)00064-5 [pii]
AID - 10.1016/j.jss.2015.01.033 [doi]
PST - ppublish
SO  - J Surg Res. 2015 May 15;195(2):481-7. doi: 10.1016/j.jss.2015.01.033. Epub 2015 
      Jan 28.