PMID- 25770896
OWN - NLM
STAT- MEDLINE
DCOM- 20150721
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1391
DP  - 2015 Apr 24
TI  - Determination of estrogenic mycotoxins in environmental water samples by 
      low-toxicity dispersive liquid-liquid microextraction and liquid 
      chromatography-tandem mass spectrometry.
PG  - 1-8
LID - S0021-9673(15)00340-4 [pii]
LID - 10.1016/j.chroma.2015.02.067 [doi]
AB  - A novel, simple, rapid and eco-friendly method based on dispersive liquid-liquid 
      microextraction using a bromosolvent was developed to determine six estrogenic 
      mycotoxins (zearalenone, zearalanone, alpha-zearalanol, beta-zearalanol, alpha-zearalenol 
      and beta-zearalenol) in water samples by liquid chromatography-electrospray 
      ionization tandem mass spectrometry in the negative mode (LC-ESI-MS/MS). The 
      optimal conditions for this method include the use of 100 muL bromocyclohexane as 
      an extraction solvent (using a non-dispersion solvent), 10 mL of aqueous sample 
      (adjusted to pH 4), a vortex extraction time of 2 min, centrifugation for 10 min 
      at 3500 rpm and no ionic strength adjustment. The calibration function was linear 
      and was verified by applying the Mandel fitting test with a 95% confidence level. 
      No matrix effect was observed. According to the relative standard deviations 
      (RSDs), the precision was better than 13% for the repeatability and intermediate 
      precision. The average recoveries of the spiked compounds ranged from 81 to 118%. 
      The method limits of detection (LOD) and quantification (LOQ) considering a 
      125-fold pre-concentration step were 4-20 and 8-40 ng L(-1), respectively. Next, 
      the method was applied to the analysis of the environmental aqueous samples, 
      demonstrating the presence of beta-zearalanol and zearalanone in the river water 
      samples.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Emidio, Elissandro Soares
AU  - Emidio ES
AD  - Analytical Chemistry Department, Chemistry Institute, Sao Paulo State 
      University-UNESP, Araraquara, SP 14801-970, Brazil.
FAU - da Silva, Claudia Pereira
AU  - da Silva CP
AD  - Analytical Chemistry Department, Chemistry Institute, Sao Paulo State 
      University-UNESP, Araraquara, SP 14801-970, Brazil.
FAU - de Marchi, Mary Rosa Rodrigues
AU  - de Marchi MR
AD  - Analytical Chemistry Department, Chemistry Institute, Sao Paulo State 
      University-UNESP, Araraquara, SP 14801-970, Brazil. Electronic address: 
      mssqam@iq.unesp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150301
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 0 (Mycotoxins)
RN  - 0 (zearalenol)
RN  - 5W827M159J (Zearalenone)
RN  - 76LO2L2V39 (Zeranol)
RN  - HUN219N434 (taleranol)
SB  - IM
MH  - Chromatography, Liquid/methods
MH  - Estrogens, Non-Steroidal/*analysis
MH  - Fresh Water/chemistry
MH  - Limit of Detection
MH  - Liquid Phase Microextraction
MH  - Mycotoxins/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/methods
MH  - Zearalenone/*analysis
MH  - Zeranol/*analogs & derivatives/*analysis
OTO - NOTNLM
OT  - Bromosolvents
OT  - Dispersive liquid-liquid microextraction
OT  - Estrogenic mycotoxins
OT  - LC-MS/MS
OT  - Water samples
EDAT- 2015/03/17 06:00
MHDA- 2015/07/22 06:00
CRDT- 2015/03/16 06:00
PHST- 2014/10/29 00:00 [received]
PHST- 2015/02/18 00:00 [revised]
PHST- 2015/02/23 00:00 [accepted]
PHST- 2015/03/16 06:00 [entrez]
PHST- 2015/03/17 06:00 [pubmed]
PHST- 2015/07/22 06:00 [medline]
AID - S0021-9673(15)00340-4 [pii]
AID - 10.1016/j.chroma.2015.02.067 [doi]
PST - ppublish
SO  - J Chromatogr A. 2015 Apr 24;1391:1-8. doi: 10.1016/j.chroma.2015.02.067. Epub 
      2015 Mar 1.