PMID- 25771180
OWN - NLM
STAT- MEDLINE
DCOM- 20150811
LR  - 20181202
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 66
IP  - 2
DP  - 2015 Aug
TI  - Characterizing the inhibitory action of zinc oxide nanoparticles on allergic-type 
      mast cell activation.
PG  - 139-46
LID - S0161-5890(15)00070-X [pii]
LID - 10.1016/j.molimm.2015.02.021 [doi]
AB  - The development of nanoparticles (NPs) for commercial products is undergoing a 
      dramatic expansion. Many sunscreens and cosmetics now use zinc oxide (ZnO) or 
      titania (TiO2) NPs, which are effective ultraviolet (UV) filters. Zinc oxide 
      topical creams are also used in mild anti-inflammatory treatments. In this study 
      we evaluated the effect of size and dispersion state of ZnO and TiO2 NPs, 
      compared to "bulk" ZnO, on mast cell degranulation and viability. ZnO and TiO2 
      NPs were characterized using dynamic light scattering and disc centrifugation. 
      Rat basophilic leukaemia (RBL-2H3) cells and primary mouse bone marrow-derived 
      mast cells (BMMCs) were exposed to ZnO and TiO2 NPs of different sizes (25-200 
      nm) and surface coatings at concentrations from 1 to 200 mug/mL. The effect of NPs 
      on immunoglobulin E (IgE)-dependent mast cell degranulation was assessed by 
      measuring release of both beta-hexosaminidase and histamine via colorimetric and 
      ELISA assays. The intracellular level of Zn(2+) and Ca(2+) ions were measured 
      using zinquin ethyl ester and Fluo-4 AM fluorescence probes, respectively. 
      Cellular viability was determined using the soluble tetrazolium-based MTS 
      colorimetric assay. Exposure of RBL-2H3 and primary mouse BMMC to ZnO NPs 
      markedly inhibited both histamine and beta-hexosaminidase release. This effect was 
      both particle size and dispersion dependent. In contrast, TiO2 NPs did not 
      inhibit the allergic response. These effects were independent of cytotoxicity, 
      which was observed only at high concentrations of ZnO NPs, and was not observed 
      for TiO2 NPs. The inhibitory effects of ZnO NPs on mast cells were inversely 
      proportional to particle size and dispersion status, and thus these NPs may have 
      greater potential than "bulk" zinc in the inhibition of allergic responses.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Feltis, B N
AU  - Feltis BN
AD  - School of Medical Sciences, RMIT University, Melbourne, Australia; Department of 
      Materials Engineering, Monash University, Melbourne, Australia. Electronic 
      address: bryce.feltis@rmit.edu.au.
FAU - Elbaz, A
AU  - Elbaz A
AD  - Faculty of Veterinary Medicine, Tripoli University, Tripoli, Libya.
FAU - Wright, P F A
AU  - Wright PF
AD  - School of Medical Sciences, RMIT University, Melbourne, Australia.
FAU - Mackay, G A
AU  - Mackay GA
AD  - Department of Pharmacology & Therapeutics, University of Melbourne, Melbourne, 
      Australia.
FAU - Turney, T W
AU  - Turney TW
AD  - Department of Materials Engineering, Monash University, Melbourne, Australia.
FAU - Lopata, A L
AU  - Lopata AL
AD  - Centre for Biodiscovery and Molecular Development of Therapeutics, James Cook 
      University, Townsville, Australia; School of Applied Sciences, RMIT University, 
      Melbourne, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150312
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cations, Divalent)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - 820484N8I3 (Histamine)
RN  - D1JT611TNE (Titanium)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - J41CSQ7QDS (Zinc)
RN  - SOI2LOH54Z (Zinc Oxide)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Basophils/cytology/*drug effects/immunology
MH  - Calcium/metabolism
MH  - Cations, Divalent
MH  - Cell Degranulation/drug effects/immunology
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Histamine/metabolism
MH  - Histamine Release/drug effects
MH  - Mast Cells/cytology/*drug effects/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Particle Size
MH  - Primary Cell Culture
MH  - Rats
MH  - Titanium/pharmacology
MH  - Zinc/metabolism
MH  - Zinc Oxide/*pharmacology
MH  - beta-N-Acetylhexosaminidases/metabolism
OTO - NOTNLM
OT  - BMMCs
OT  - Cytotoxicity
OT  - Histamine
OT  - RBL-2H3
OT  - TiO(2)
OT  - ZnO
OT  - beta-Hexosaminidase
EDAT- 2015/03/17 06:00
MHDA- 2015/08/12 06:00
CRDT- 2015/03/16 06:00
PHST- 2014/12/13 00:00 [received]
PHST- 2015/02/11 00:00 [revised]
PHST- 2015/02/19 00:00 [accepted]
PHST- 2015/03/16 06:00 [entrez]
PHST- 2015/03/17 06:00 [pubmed]
PHST- 2015/08/12 06:00 [medline]
AID - S0161-5890(15)00070-X [pii]
AID - 10.1016/j.molimm.2015.02.021 [doi]
PST - ppublish
SO  - Mol Immunol. 2015 Aug;66(2):139-46. doi: 10.1016/j.molimm.2015.02.021. Epub 2015 
      Mar 12.