PMID- 25772000 OWN - NLM STAT- MEDLINE DCOM- 20160525 LR - 20150828 IS - 1552-4965 (Electronic) IS - 1549-3296 (Linking) VI - 103 IP - 10 DP - 2015 Oct TI - Osteogenesis and chondrogenesis of biomimetic integrated porous PVA/gel/V-n-HA/pa6 scaffolds and BMSCs construct in repair of articular osteochondral defect. PG - 3226-36 LID - 10.1002/jbm.a.35452 [doi] AB - A novel bi-layered osteochondral scaffold, including of PVA/Gel/V layer for the cartilage and n-HA/PA6 layer for the subchondral bone, has been proposed to evaluate the potential of the engineered of osteochondral grafts in repairing articular osteochondral defects in rabbits. The two different layers of the scaffolds were seeded with allogenic bone marrow-derived stem cells (BMSCs), which were chondrogenically and osteogenically induced respectively. The critical-size osteochondral defects were created in the knees of adult rabbits. The defects were treated with cell-bi-layered constructs (Group A), bi-layered constructs (Group B) and untreated group C as control group. The adhesion, proliferation and differentiation of BMSCs were demonstrated by immunohistochemical staining and scanning electron microscopy (SEM) in vitro. Cell survival was tracked via fluorescent labeling in vivo. Overall, the porous PVA/Gel/V-n-HA/PA6 scaffold was compatible and had no negative effects on the BMSCs in vitro culture. The cell-bi-layered scaffolds showed superior repair results as compared to the control group using gross examination and histological assessment. With BMSCs implantation, the two different layers of the composite biomimetic scaffolds provided a suitable environment for cells to form respective tissue. Simultaneously, the RT-PCR results confirmed the expression of specific extracellular matrix (ECM) markers for cartilaginous or osteoid tissue. This investigation showed that the porous PVA/Gel/V-n-HA/PA6 scaffold is a potential matrix for treatment of osteochondral defects, and the method of using chondrogenically and osteogenically differentiated BMSCs as seed cells on each layer might be a promising strategy in repair of articular osteochondral defect due to enhanced chondrogenesis and osteogenesis. CI - (c) 2015 Wiley Periodicals, Inc. FAU - Li, Xiang AU - Li X AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. FAU - Li, Yubao AU - Li Y AD - Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, 610064, China. FAU - Zuo, Yi AU - Zuo Y AD - Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, 610064, China. FAU - Qu, Dan AU - Qu D AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. FAU - Liu, Yiming AU - Liu Y AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. FAU - Chen, Tao AU - Chen T AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. FAU - Jiang, Nan AU - Jiang N AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. FAU - Li, Hui AU - Li H AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. FAU - Li, Jihua AU - Li J AD - State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150401 PL - United States TA - J Biomed Mater Res A JT - Journal of biomedical materials research. Part A JID - 101234237 SB - IM MH - Allografts MH - Animals MH - Bone Marrow Cells/*metabolism/ultrastructure MH - *Bone Marrow Transplantation MH - *Chondrogenesis MH - Knee Injuries/metabolism/pathology/*therapy MH - *Osteogenesis MH - Rabbits MH - Stem Cells/*metabolism/ultrastructure MH - Tissue Scaffolds/*chemistry OTO - NOTNLM OT - BMSCs OT - bilayered biomimetic scaffolds OT - chondrogenesis OT - osteochondral defect OT - osteogenesis EDAT- 2015/03/17 06:00 MHDA- 2016/05/26 06:00 CRDT- 2015/03/17 06:00 PHST- 2014/11/28 00:00 [received] PHST- 2015/02/23 00:00 [revised] PHST- 2015/02/27 00:00 [accepted] PHST- 2015/03/17 06:00 [entrez] PHST- 2015/03/17 06:00 [pubmed] PHST- 2016/05/26 06:00 [medline] AID - 10.1002/jbm.a.35452 [doi] PST - ppublish SO - J Biomed Mater Res A. 2015 Oct;103(10):3226-36. doi: 10.1002/jbm.a.35452. Epub 2015 Apr 1.