PMID- 2577369
OWN - NLM
STAT- MEDLINE
DCOM- 19910516
LR  - 20131121
IS  - 1043-4674 (Print)
IS  - 1043-4674 (Linking)
VI  - 1
IP  - 2
DP  - 1989 Nov
TI  - Evidence for a single glutamate receptor of the ionotropic kainate/quisqualate 
      type.
PG  - 153-8
AB  - The kainate (KA) and the quisqualate (QUIS) receptors that activate cation 
      channels in the central nervous system have previously been defined as two of the 
      major glutamate receptor types. In amphibian brain, an exceptionally rich source 
      of these sites, they can be coextracted by octylglucoside and shown to behave as 
      one entity in all analyses made in solution. When partly purified by lectin 
      affinity, ion-exchange chromatography or by sucrose gradient centrifugation, the 
      two activities comigrate in a 1:1 ratio. When the QUIS component is bound to an 
      immobilized specific QUIS agonist, the KA component is extracted in parallel with 
      it. There are equivalent numbers of the QUIS and KA sites and the two sites show 
      a single affinity series for the binding of glutamatergic agonists. We deduce 
      that KA or QUIS select different conformations of a single KA/QUIS receptor 
      binding site, leading thus to the different channel-opening events that have been 
      reported for these two agonists.
FAU - Henley, J M
AU  - Henley JM
AD  - MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, England.
FAU - Ambrosini, A
AU  - Ambrosini A
FAU - Krogsgaard-Larsen, P
AU  - Krogsgaard-Larsen P
FAU - Barnard, E A
AU  - Barnard EA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - New Biol
JT  - The New biologist
JID - 9000976
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Receptors, Kainic Acid)
RN  - 0 (Receptors, Neurotransmitter)
RN  - 2552-55-8 (Ibotenic Acid)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
RN  - 8OC22C1B99 (Quisqualic Acid)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione
MH  - Animals
MH  - *Brain Chemistry
MH  - Ibotenic Acid/*analogs & derivatives/metabolism/pharmacology
MH  - Ion Channel Gating/drug effects
MH  - Kainic Acid/*metabolism/pharmacology
MH  - Kinetics
MH  - Protein Conformation
MH  - Quinoxalines/pharmacology
MH  - Quisqualic Acid/*metabolism/pharmacology
MH  - Receptors, AMPA
MH  - Receptors, Glutamate
MH  - Receptors, Kainic Acid
MH  - Receptors, Neurotransmitter/*classification/drug effects/isolation & 
      purification/metabolism
MH  - Xenopus laevis
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
PST - ppublish
SO  - New Biol. 1989 Nov;1(2):153-8.