PMID- 25773901
OWN - NLM
STAT- MEDLINE
DCOM- 20160216
LR  - 20150429
IS  - 1556-4029 (Electronic)
IS  - 0022-1198 (Linking)
VI  - 60
IP  - 3
DP  - 2015 May
TI  - Characterization of protein alterations in damaged axons in the brainstem 
      following traumatic brain injury using fourier transform infrared 
      microspectroscopy: a preliminary study.
PG  - 759-63
LID - 10.1111/1556-4029.12743 [doi]
AB  - Axonal injury contributes greatly to neurological dysfunction following traumatic 
      brain injury (TBI), but current histological diagnostic methods are limited in 
      identifying the pathological profiles of injured axons and unable to provide an 
      objective and accurate quantification. Fourier transform infrared 
      microspectroscopy (FTIRM) has the ability to offer macromolecular bioinformatics 
      of the tissues including biochemical composition and structure by calculating 
      band absorption intensity. In this study, axonal injury in the brainstem of rats 
      with traumatic brain injury at 72 h post-trauma, which was confirmed with 
      beta-amyloid precursor protein (beta-APP) immunostaining, was detected with FTIRM 
      technique. The lower intensity of infrared absorbance under the amide I band 
      corresponds strongly to the area of axonal injury, and further analysis of amide 
      I band shows significant differences in protein conformation between injured and 
      normal axons. The findings indicate that using FTIRM technique, the amide I band 
      has potentials to be a infrared spectral marker of axonal injury.
CI  - (c) 2015 American Academy of Forensic Sciences.
FAU - Zhang, Ji
AU  - Zhang J
AD  - Department of Forensic Medicine, Tongji Medical College of Huazhong University of 
      Science and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.
FAU - Niu, Fei
AU  - Niu F
FAU - Dong, Hongmei
AU  - Dong H
FAU - Liu, Liang
AU  - Liu L
FAU - Li, Jie
AU  - Li J
FAU - Li, Shangxun
AU  - Li S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150313
PL  - United States
TA  - J Forensic Sci
JT  - Journal of forensic sciences
JID - 0375370
RN  - 0 (Amides)
RN  - 0 (Amyloid beta-Protein Precursor)
SB  - IM
MH  - Amides/chemistry
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Animals
MH  - Brain Injuries/*metabolism/pathology
MH  - Brain Stem/cytology
MH  - Diffuse Axonal Injury/*metabolism/pathology
MH  - Forensic Pathology
MH  - Immunohistochemistry
MH  - Protein Conformation
MH  - Rats, Sprague-Dawley
MH  - *Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - Fourier transform infrared microspectroscopy
OT  - axonal injury
OT  - forensic pathology
OT  - forensic science
OT  - protein conformation
OT  - traumatic brain injury
EDAT- 2015/03/17 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/03/17 06:00
PHST- 2014/02/13 00:00 [received]
PHST- 2014/06/04 00:00 [revised]
PHST- 2014/06/08 00:00 [accepted]
PHST- 2015/03/17 06:00 [entrez]
PHST- 2015/03/17 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - 10.1111/1556-4029.12743 [doi]
PST - ppublish
SO  - J Forensic Sci. 2015 May;60(3):759-63. doi: 10.1111/1556-4029.12743. Epub 2015 
      Mar 13.