PMID- 25779928
OWN - NLM
STAT- MEDLINE
DCOM- 20160115
LR  - 20220318
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 1
DP  - 2015 Jul
TI  - Trop2 inhibition suppresses the proliferation and invasion of laryngeal carcinoma 
      cells via the extracellular signal-regulated kinase/mitogen-activated protein 
      kinase pathway.
PG  - 865-70
LID - 10.3892/mmr.2015.3485 [doi]
AB  - The cell surface glycoprotein Trop2 is overexpressed in various types of 
      epithelial cancer. Laryngeal carcinoma is one of the most common types of head 
      and neck cancer and in a previous study, the expression of Trop2 in laryngeal 
      squamous cell carcinoma (LSCC) was identified as an independent prognostic 
      factor. However, the biological significance of Trop2 in LSCC development remains 
      unclear. In the current study, Trop2 protein expression in fresh LSCC tissue and 
      paracancerous tissue was investigated using western blotting. Trop2 in the Hep2 
      laryngeal cell line was subsequently suppressed by transfection with small 
      interfering RNA (siRNA). The effects of knockdown of Trop2 on cell viability, 
      migration, invasiveness and ERK/MAPK pathway activity were investigated in the 
      current study. The expression of Trop2 in fresh LSCC tissue was demonstrated to 
      be significantly greater than that in paracancerous tissue. Trop2 expression was 
      also identified to be required for proliferation, migration and invasiveness of 
      Hep2 laryngeal carcinoma cells, as all were blocked by siRNA-mediated Trop2 
      inhibition. Notably, the ERK/MAPK signaling pathway and cell cycle factor, cyclin 
      D1, were identified to be suppressed following the knockdown of Trop2 in Hep2 
      cells. These observations suggest that Trop2 serves an oncogenic role in LSCC and 
      has potential as a therapeutic target.
FAU - Wang, Xu-Dong
AU  - Wang XD
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nantong University, 
      Nantong, Jiangsu 226000, P.R. China.
FAU - Wang, Qiang
AU  - Wang Q
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of 
      Nantong University, Nantong, Jiangsu 226000, P.R. China.
FAU - Chen, Xiao-Lin
AU  - Chen XL
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of 
      Nantong University, Nantong, Jiangsu 226000, P.R. China.
FAU - Huang, Jian-Fei
AU  - Huang JF
AD  - Department of Pathology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226000, P.R. China.
FAU - Yin, Yong
AU  - Yin Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of 
      Nantong University, Nantong, Jiangsu 226000, P.R. China.
FAU - Da, Peng
AU  - Da P
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of 
      Nantong University, Nantong, Jiangsu 226000, P.R. China.
FAU - Wu, Hao
AU  - Wu H
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of 
      Nantong University, Nantong, Jiangsu 226000, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150313
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (TACSTD2 protein, human)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
SB  - IM
MH  - Antigens, Neoplasm/biosynthesis/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Adhesion Molecules/antagonists & inhibitors/biosynthesis/*genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Cyclin D1/biosynthesis/genetics
MH  - Extracellular Signal-Regulated MAP Kinases/biosynthesis/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Mitogen-Activated Protein Kinase 1/biosynthesis/genetics
MH  - Neoplasm Invasiveness/genetics
MH  - Signal Transduction/*genetics
PMC - PMC4438912
EDAT- 2015/03/18 06:00
MHDA- 2016/01/16 06:00
PMCR- 2015/03/13
CRDT- 2015/03/18 06:00
PHST- 2014/04/09 00:00 [received]
PHST- 2015/01/22 00:00 [accepted]
PHST- 2015/03/18 06:00 [entrez]
PHST- 2015/03/18 06:00 [pubmed]
PHST- 2016/01/16 06:00 [medline]
PHST- 2015/03/13 00:00 [pmc-release]
AID - mmr-12-01-0865 [pii]
AID - 10.3892/mmr.2015.3485 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Jul;12(1):865-70. doi: 10.3892/mmr.2015.3485. Epub 2015 Mar 13.