PMID- 25780270
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 0008-6223 (Print)
IS  - 0008-6223 (Linking)
VI  - 78
DP  - 2014 Nov 1
TI  - Multi-walled carbon nanotube length as a critical determinant of bioreactivity 
      with primary human pulmonary alveolar cells.
PG  - 26-37
AB  - Multiwalled carbon nanotube (MWCNT) length is suggested to critically determine 
      their pulmonary toxicity. This stems from in vitro and in vivo rodent studies and 
      in vitro human studies using cell lines (typically cancerous). There is little 
      data using primary human lung cells. We addressed this knowledge gap, using 
      highly relevant, primary human alveolar cell models exposed to precisely 
      synthesized and thoroughly characterized MWCNTs. In this work, transformed human 
      alveolar type-I-like epithelial cells (TT1), primary human alveolar type-II 
      epithelial cells (ATII) and alveolar macrophages (AM) were treated with 
      increasing concentrations of MWCNTs before measuring cytotoxicity, inflammatory 
      mediator release and MAP kinase signalling. Strikingly, we observed that short 
      MWCNTs (~0.6 microm in length) induced significantly greater responses from the 
      epithelial cells, whilst AM were particularly susceptible to long MWCNTs (~20 
      microm). These differences in the pattern of mediator release were associated with 
      alternative profiles of JNK, p38 and ERK1/2 MAP kinase signal transduction within 
      each cell type. This study, using highly relevant target human alveolar cells and 
      well defined and characterized MWCNTs, shows marked cellular responses to the 
      MWCNTs that vary according to the target cell type, as well as the aspect ratio 
      of the MWCNT.
FAU - Sweeney, Sinbad
AU  - Sweeney S
AD  - Lung Cell Biology, Section of Pharmacology and Toxicology, National Heart and 
      Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK.
FAU - Berhanu, Deborah
AU  - Berhanu D
AD  - Physical Sciences Department, Kingsborough Community College, City University of 
      New York, 2001 Oriental Boulevard, New York, NY 11235 ; Earth Sciences, Natural 
      History Museum, Cromwell Road, London SW7 5BD, UK.
FAU - Misra, Superb K
AU  - Misra SK
AD  - School of Geography, Earth and Environmental Sciences, University of Birmingham, 
      Edgbaston, Birmingham, B5 2TT, UK ; Earth Sciences, Natural History Museum, 
      Cromwell Road, London SW7 5BD, UK.
FAU - Thorley, Andrew J
AU  - Thorley AJ
AD  - Lung Cell Biology, Section of Pharmacology and Toxicology, National Heart and 
      Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK.
FAU - Valsami-Jones, Eugenia
AU  - Valsami-Jones E
AD  - School of Geography, Earth and Environmental Sciences, University of Birmingham, 
      Edgbaston, Birmingham, B5 2TT, UK ; Earth Sciences, Natural History Museum, 
      Cromwell Road, London SW7 5BD, UK.
FAU - Tetley, Teresa D
AU  - Tetley TD
AD  - Lung Cell Biology, Section of Pharmacology and Toxicology, National Heart and 
      Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK.
LA  - eng
GR  - U19 ES019536/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Carbon N Y
JT  - Carbon
JID - 101204394
PMC - PMC4357847
MID - NIHMS665872
EDAT- 2015/03/18 06:00
MHDA- 2015/03/18 06:01
PMCR- 2015/11/01
CRDT- 2015/03/18 06:00
PHST- 2015/03/18 06:00 [entrez]
PHST- 2015/03/18 06:00 [pubmed]
PHST- 2015/03/18 06:01 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - 10.1016/j.carbon.2014.06.033 [doi]
PST - ppublish
SO  - Carbon N Y. 2014 Nov 1;78:26-37. doi: 10.1016/j.carbon.2014.06.033.