PMID- 25780291
OWN - NLM
STAT- MEDLINE
DCOM- 20151015
LR  - 20200914
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 21
IP  - 10
DP  - 2015 Mar 14
TI  - Heme oxygenase-1 protects rat liver against warm ischemia/reperfusion injury via 
      TLR2/TLR4-triggered signaling pathways.
PG  - 2937-48
LID - 10.3748/wjg.v21.i10.2937 [doi]
AB  - AIM: To investigate the efficacy and molecular mechanisms of induced heme 
      oxygenase (HO)-1 in protecting liver from warm ischemia/reperfusion (I/R) injury. 
      METHODS: Partial warm ischemia was produced in the left and middle hepatic lobes 
      of SD rats for 75 min, followed by 6 h of reperfusion. Rats were treated with 
      saline, cobalt protoporphyrin (CoPP) or zinc protoporphyrin (ZnPP) at 24 h prior 
      to the ischemia insult. Blood and samples of ischemic lobes subjected to ischemia 
      were collected at 6 h after reperfusion. Serum transaminases level, plasma 
      lactate dehydrogenase and myeloperoxidase activity in liver were measured. Liver 
      histological injury and inflammatory cell infiltration were evaluated by tissue 
      section and liver immunohistochemical analysis. We used quantitative reverse 
      transcription polymerase chain reaction to analyze liver expression of 
      inflammatory cytokines and chemokines. The cell lysates were subjected to 
      immunoprecipitation with anti-Toll-IL-1R-containing adaptor inducing interferon-beta 
      (TRIF) and anti-myeloid differentiation factor 88 (MyD88), and then the 
      immunoprecipitates were analyzed by SDS-PAGE and immunoblotted with the indicated 
      antibodies. RESULTS: HO-1 protected livers from I/R injury, as evidenced by 
      diminished liver enzymes and well-preserved tissue architecture. In comparison 
      with ZnPP livers 6 h after surgery, CoPP treatment livers showed a significant 
      increase inflammatory cell infiltration of lymphocytes, plasma cells, neutrophils 
      and macrophages. The Toll-like receptor (TLR)-4 and TANK binding kinase 1 protein 
      levels of rats treated with CoPP significantly reduced in TRIF-immunoprecipitated 
      complex, as compared with ZnPP treatment. In addition, pretreatment with CoPP 
      reduced the expression levels of TLR2, TLR4, IL-1R-associated kinase (IRAK)-1 and 
      tumor necrosis factor receptor-associated factor 6 in MyD88-immunoprecipitated 
      complex. The inflammatory cytokines and chemokines mRNA expression rapidly 
      decreased in CoPP-pretreated liver, compared with the ZnPP-treated group. 
      However, the expression of negative regulators Toll-interacting protein, 
      suppressor of cytokine signaling-1, IRAK-M and Src homology 2 domain-containing 
      inositol-5-phosphatase-1 in CoPP treatment rats were markedly up-regulated as 
      compared with ZnPP-treated rats. CONCLUSION: HO-1 protects liver against I/R 
      injury by inhibiting TLR2/TLR4-triggered MyD88- and TRIF-dependent signaling 
      pathways and increasing expression of negative regulators of TLR signaling in 
      rats.
FAU - Huang, Han-Fei
AU  - Huang HF
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Zeng, Zhong
AU  - Zeng Z
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Wang, Kun-Hua
AU  - Wang KH
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Zhang, Hai-Yan
AU  - Zhang HY
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Wang, Shuai
AU  - Wang S
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Zhou, Wen-Xiang
AU  - Zhou WX
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Wang, Zhan-Bo
AU  - Wang ZB
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Xu, Wang-Gang
AU  - Xu WG
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
FAU - Duan, Jian
AU  - Duan J
AD  - Han-Fei Huang, Zhong Zeng, Kun-Hua Wang, Hai-Yan Zhang, Shuai Wang, Wen-Xiang 
      Zhou, Zhan-Bo Wang, Wang-Gang Xu, Jian Duan, Organ Transplantation Center, the 
      First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan 
      Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Protoporphyrins)
RN  - 0 (Ticam1 protein, rat)
RN  - 0 (Tlr2 protein, rat)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 15442-64-5 (zinc protoporphyrin)
RN  - 63AAN3JDZE (cobaltiprotoporphyrin)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Hmox1 protein, rat)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport/metabolism
MH  - Animals
MH  - Cytoprotection
MH  - Disease Models, Animal
MH  - Enzyme Inhibitors/pharmacology
MH  - Heme Oxygenase (Decyclizing)/antagonists & inhibitors/*metabolism
MH  - Liver/*drug effects/enzymology/immunology/pathology
MH  - Male
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Protoporphyrins/*pharmacology
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/enzymology/etiology/immunology/pathology/*prevention & control
MH  - Signal Transduction/*drug effects
MH  - Toll-Like Receptor 2/*drug effects/metabolism
MH  - Toll-Like Receptor 4/*drug effects/metabolism
MH  - Warm Ischemia/*adverse effects
PMC - PMC4356913
OTO - NOTNLM
OT  - Heme oxygenase-1
OT  - Ischemia reperfusion injury
OT  - Liver
OT  - Myeloid differentiation factor 88
OT  - Toll-like receptor
EDAT- 2015/03/18 06:00
MHDA- 2015/10/16 06:00
PMCR- 2015/03/14
CRDT- 2015/03/18 06:00
PHST- 2014/07/03 00:00 [received]
PHST- 2014/08/09 00:00 [revised]
PHST- 2014/11/07 00:00 [accepted]
PHST- 2015/03/18 06:00 [entrez]
PHST- 2015/03/18 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
PHST- 2015/03/14 00:00 [pmc-release]
AID - 10.3748/wjg.v21.i10.2937 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2015 Mar 14;21(10):2937-48. doi: 10.3748/wjg.v21.i10.2937.