PMID- 25780695
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150317
LR  - 20201001
IS  - 2093-6966 (Print)
IS  - 2234-6856 (Electronic)
IS  - 2093-6966 (Linking)
VI  - 17
IP  - 2
DP  - 2014 Jun
TI  - Study on a 4-week recovery test of sweet bee venom after a 13-week, repeated, 
      intramuscular dose toxicity test in sprague-dawley rats.
PG  - 18-26
LID - 10.3831/KPI.2014.17.012 [doi]
AB  - OBJECTIVES: This study was performed to check for reversibility in the changes 
      induced by a 13-week, repeated, dose toxicity test of Sweet Bee Venom (SBV) in 
      Sprague-Dawley (SD) rats. METHODS: Fifteen male and 15 female SD rats were 
      treated with 0.28 mg/kg of SBV (high-dosage group) and the same numbers of male 
      and female SD rats were treated with 0.2 mL/kg of normal saline (control group) 
      for 13 weeks. We selected five male and five female SD rats from the high-dosage 
      group and the same numbers of male and female SD rats from the control group, and 
      we observed these rats for four weeks. We conducted body-weight measurements, 
      ophthalmic examinations, urinalyses and hematology, biochemistry, histology 
      tests. RESULTS: (1) Hyperemia and movement disorder were observed in the 13-week, 
      repeated, dose toxicity test, but these symptoms were not observed during the 
      recovery period. (2) The rats in the high-dose group showed no significant 
      changes in weight compared to the control group. (3) No significant differences 
      in the ophthalmic parameters, urine analyses, complete blood cell counts (CBCs), 
      and biochemistry were observed among the recovery groups. (4) No changes in organ 
      weights were observed during the recovery period. (5) Histological examination of 
      the thigh muscle indicated cell infiltration, inflammation, degeneration, 
      necrosis of muscle fiber, and fibrosis during the treatment period, but these 
      changes were not observed during the recovery period. The fatty liver change that 
      was observed during the toxicity test was not observed during the recovery 
      period. No other organ abnormalities were observed. CONCLUSION: The changes that 
      occurred during the 13-week, repeated, dose toxicity test are reversible, and SBV 
      can be safely used as a treatment modality.
FAU - Kang, Hyunmin
AU  - Kang H
AD  - Department of Acupuncture & Moxibustion Medicine, Sangji University College of 
      Oriental Medicine, Wonju, Korea.
FAU - Lim, Chungsan
AU  - Lim C
AD  - Department of Acupuncture & Moxibustion Medicine, Sangji University College of 
      Oriental Medicine, Wonju, Korea.
FAU - Lee, Seungbae
AU  - Lee S
AD  - Division of Animal Resources and Life Science, Sangji University, Wonju, Korea.
FAU - Kim, Byoungwoo
AU  - Kim B
AD  - Department of Internal Medicine, Sangji University College of Oriental Medicine, 
      Wonju, Korea.
FAU - Kwon, Kirok
AU  - Kwon K
AD  - Research Center of the Korean Pharmacopuncture Institute, Seoul, Korea.
FAU - Lee, Kwangho
AU  - Lee K
AD  - Department of Acupuncture & Moxibustion Medicine, Sangji University College of 
      Oriental Medicine, Wonju, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Pharmacopuncture
JT  - Journal of pharmacopuncture
JID - 101572812
PMC - PMC4332000
OTO - NOTNLM
OT  - 13-week repeated dose toxicity
OT  - 4-week recovery test
OT  - Sweet Bee Venom
OT  - acupuncture
OT  - melittin
OT  - pharmacopuncture
COIS- Conflict of interest The authors declare that there are no conflicts of interest.
EDAT- 2015/03/18 06:00
MHDA- 2015/03/18 06:01
PMCR- 2014/06/01
CRDT- 2015/03/18 06:00
PHST- 2013/11/07 00:00 [received]
PHST- 2014/02/12 00:00 [accepted]
PHST- 2015/03/18 06:00 [entrez]
PHST- 2015/03/18 06:00 [pubmed]
PHST- 2015/03/18 06:01 [medline]
PHST- 2014/06/01 00:00 [pmc-release]
AID - 10.3831/KPI.2014.17.012 [doi]
PST - ppublish
SO  - J Pharmacopuncture. 2014 Jun;17(2):18-26. doi: 10.3831/KPI.2014.17.012.