PMID- 25781330
OWN - NLM
STAT- MEDLINE
DCOM- 20151201
LR  - 20231104
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - Spontaneous and divergent hexaploid triticales derived from common wheat x rye by 
      complete elimination of D-genome chromosomes.
PG  - e0120421
LID - 10.1371/journal.pone.0120421 [doi]
LID - e0120421
AB  - BACKGROUND: Hexaploid triticale could be either synthesized by crossing 
      tetraploid wheat with rye, or developed by crossing hexaploid wheat with a 
      hexaploid triticale or an octoploid triticale. METHODOLOGY/PRINCIPAL FINDINGS: 
      Here two hexaploid triticales with great morphologic divergence derived from 
      common wheat cultivar M8003 (Triticum aestivum L.) x Austrian rye (Secale cereale 
      L.) were reported, exhibiting high resistance for powdery mildew and stripe rust 
      and potential for wheat improvement. Sequential fluorescence in situ 
      hybridization (FISH) and genomic in situ hybridization (GISH) karyotyping 
      revealed that D-genome chromosomes were completely eliminated and the whole 
      A-genome, B-genome and R-genome chromosomes were retained in both lines. 
      Furthermore, plentiful alterations of wheat chromosomes including 5A and 7B were 
      detected in both triticales and additionally altered 5B, 7A chromosome and 
      restructured chromosome 2A was assayed in N9116H and N9116M, respectively, even 
      after selfing for several decades. Besides, meiotic asynchrony was displayed and 
      a variety of storage protein variations were assayed, especially in the 
      HMW/LMW-GS region and secalins region in both triticales. CONCLUSION: This study 
      confirms that whole D-genome chromosomes could be preferentially eliminated in 
      the hybrid of common wheat x rye, "genome shock" was accompanying the 
      allopolyploidization of nascent triticales, and great morphologic divergence 
      might result from the genetic variations. Moreover, new hexaploid triticale lines 
      contributing potential resistance resources for wheat improvement were produced.
FAU - Li, Hao
AU  - Li H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A & F University, Yangling, Shaanxi, China.
FAU - Guo, Xiaoxue
AU  - Guo X
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A & F University, Yangling, Shaanxi, China.
FAU - Wang, Changyou
AU  - Wang C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A & F University, Yangling, Shaanxi, China.
FAU - Ji, Wanquan
AU  - Ji W
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of 
      Agronomy, Northwest A & F University, Yangling, Shaanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150317
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 8002-80-0 (Glutens)
SB  - IM
MH  - *Abnormal Karyotype
MH  - Chimera/*genetics
MH  - Chromosomes, Plant/*genetics
MH  - *Genome, Plant
MH  - Glutens/genetics
MH  - *Polyploidy
MH  - Secale/*genetics
MH  - Triticum/*genetics
PMC - PMC4364014
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/03/18 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/03/17
CRDT- 2015/03/18 06:00
PHST- 2014/09/18 00:00 [received]
PHST- 2015/01/22 00:00 [accepted]
PHST- 2015/03/18 06:00 [entrez]
PHST- 2015/03/18 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/03/17 00:00 [pmc-release]
AID - PONE-D-14-42007 [pii]
AID - 10.1371/journal.pone.0120421 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 17;10(3):e0120421. doi: 10.1371/journal.pone.0120421. 
      eCollection 2015.