PMID- 25783173 OWN - NLM STAT- MEDLINE DCOM- 20160704 LR - 20181202 IS - 1720-8319 (Electronic) IS - 1594-0667 (Linking) VI - 27 IP - 6 DP - 2015 Dec TI - Aging does not affect the ability of human monocyte-derived dendritic cells to phagocytose Candida albicans. PG - 785-9 LID - 10.1007/s40520-015-0344-1 [doi] AB - BACKGROUND: Dendritic cells (DCs) are the most potent antigen-presenting cells, playing a key role in induction of both innate and adaptive immunity. Immunosenescence refers to age-associated changes in the immune system, which may be associated with susceptibility to infections and their clinical complications. The precise effects of aging on DCs in immunity to infections are not well understood. Among the common pathogenic microorganisms, the fungus Candida albicans is an important pathogen for the development of invasive infections, especially in immunocompromised individuals, as well as during aging. AIMS: To make a comparative in vitro evaluation of the immunomodulatory function of DCs challenged with C. albicans, by phagocytosis of the fungal cells, and determine the involvement of TLR2 and TLR4 receptors. For this purpose, DCs were generated with the use of peripheral blood monocytes from healthy young and aged subjects. RESULTS: The phagocytosis of C. albicans is developed by DCs in TLR2- and TLR4-dependent way. This mechanism is not affected by aging. CONCLUSION: Given the important role of the DCs in responses against the fungus, it is evident that if changes in phagocytosis occurred with aging, impairment in the elderly could develop. However, the evidence that phagocytosis of this fungus by DCs is not impaired with aging, brings us to the question of which are the mechanisms truly associated with the prevalence of certain diseases in the elderly. FAU - do Nascimento, Magda Paula Pereira AU - do Nascimento MP AD - Department of Stomatology, Bauru School of Dentistry, University of Sao Paulo, Al. Dr. Octavio Pinheiro Brisola, 9-75, Vila Universitaria, Bauru, SP, 17012-901, Brazil. FAU - Pinke, Karen Henriette AU - Pinke KH AD - Department of Stomatology, Bauru School of Dentistry, University of Sao Paulo, Al. Dr. Octavio Pinheiro Brisola, 9-75, Vila Universitaria, Bauru, SP, 17012-901, Brazil. FAU - Penitenti, Marcimara AU - Penitenti M AD - Flow Cytometry Laboratory, Amaral Carvalho Foundation, R. Dona Silveria, 150, Centro, Jau, SP, 17210-080, Brazil. FAU - Ikoma, Maura Rosane Valerio AU - Ikoma MR AD - Flow Cytometry Laboratory, Amaral Carvalho Foundation, R. Dona Silveria, 150, Centro, Jau, SP, 17210-080, Brazil. FAU - Lara, Vanessa Soares AU - Lara VS AD - Department of Stomatology, Bauru School of Dentistry, University of Sao Paulo, Al. Dr. Octavio Pinheiro Brisola, 9-75, Vila Universitaria, Bauru, SP, 17012-901, Brazil. vanessa@fob.usp.br. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150318 PL - Germany TA - Aging Clin Exp Res JT - Aging clinical and experimental research JID - 101132995 RN - 0 (Toll-Like Receptor 2) RN - 0 (Toll-Like Receptor 4) SB - IM MH - Adult MH - Aged MH - Aging/*immunology MH - Candida albicans/*physiology MH - Cells, Cultured MH - Dendritic Cells/*immunology MH - Humans MH - Immunity/*physiology MH - Middle Aged MH - Monocytes/immunology MH - Phagocytosis/*immunology MH - Toll-Like Receptor 2/immunology MH - Toll-Like Receptor 4/immunology OTO - NOTNLM OT - C. albicans OT - Dendritic cell OT - Immunosenescence OT - Phagocytosis and Toll like-receptors EDAT- 2015/03/19 06:00 MHDA- 2016/07/05 06:00 CRDT- 2015/03/19 06:00 PHST- 2014/09/18 00:00 [received] PHST- 2015/02/24 00:00 [accepted] PHST- 2015/03/19 06:00 [entrez] PHST- 2015/03/19 06:00 [pubmed] PHST- 2016/07/05 06:00 [medline] AID - 10.1007/s40520-015-0344-1 [pii] AID - 10.1007/s40520-015-0344-1 [doi] PST - ppublish SO - Aging Clin Exp Res. 2015 Dec;27(6):785-9. doi: 10.1007/s40520-015-0344-1. Epub 2015 Mar 18.