PMID- 25783786
OWN - NLM
STAT- MEDLINE
DCOM- 20160203
LR  - 20181113
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 23
IP  - 2
DP  - 2015 Jun
TI  - Canine urothelial carcinoma: genomically aberrant and comparatively relevant.
PG  - 311-31
LID - 10.1007/s10577-015-9471-y [doi]
AB  - Urothelial carcinoma (UC), also referred to as transitional cell carcinoma (TCC), 
      is the most common bladder malignancy in both human and canine populations. In 
      human UC, numerous studies have demonstrated the prevalence of chromosomal 
      imbalances. Although the histopathology of the disease is similar in both 
      species, studies evaluating the genomic profile of canine UC are lacking, 
      limiting the discovery of key comparative molecular markers associated with 
      driving UC pathogenesis. In the present study, we evaluated 31 primary canine UC 
      biopsies by oligonucleotide array comparative genomic hybridization (oaCGH). 
      Results highlighted the presence of three highly recurrent numerical aberrations: 
      gain of dog chromosome (CFA) 13 and 36 and loss of CFA 19. Regional gains of CFA 
      13 and 36 were present in 97 % and 84 % of cases, respectively, and losses on CFA 
      19 were present in 77 % of cases. Fluorescence in situ hybridization (FISH), 
      using targeted bacterial artificial chromosome (BAC) clones and custom Agilent 
      SureFISH probes, was performed to detect and quantify these regions in 
      paraffin-embedded biopsy sections and urine-derived urothelial cells. The data 
      indicate that these three aberrations are potentially diagnostic of UC. 
      Comparison of our canine oaCGH data with that of 285 human cases identified a 
      series of shared copy number aberrations. Using an informatics approach to 
      interrogate the frequency of copy number aberrations across both species, we 
      identified those that had the highest joint probability of association with UC. 
      The most significant joint region contained the gene PABPC1, which should be 
      considered further for its role in UC progression. In addition, cross-species 
      filtering of genome-wide copy number data highlighted several genes as 
      high-profile candidates for further analysis, including CDKN2A, S100A8/9, and 
      LRP1B. We propose that these common aberrations are indicative of an 
      evolutionarily conserved mechanism of pathogenesis and harbor genes key to 
      urothelial neoplasia, warranting investigation for diagnostic, prognostic, and 
      therapeutic applications.
FAU - Shapiro, S G
AU  - Shapiro SG
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, 
      USA.
FAU - Raghunath, S
AU  - Raghunath S
FAU - Williams, C
AU  - Williams C
FAU - Motsinger-Reif, A A
AU  - Motsinger-Reif AA
FAU - Cullen, J M
AU  - Cullen JM
FAU - Liu, T
AU  - Liu T
FAU - Albertson, D
AU  - Albertson D
FAU - Ruvolo, M
AU  - Ruvolo M
FAU - Bergstrom Lucas, A
AU  - Bergstrom Lucas A
FAU - Jin, J
AU  - Jin J
FAU - Knapp, D W
AU  - Knapp DW
FAU - Schiffman, J D
AU  - Schiffman JD
FAU - Breen, M
AU  - Breen M
LA  - eng
GR  - T35 OD011070/OD/NIH HHS/United States
GR  - T35 RR025837/RR/NCRR NIH HHS/United States
GR  - T35/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150318
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Animals
MH  - Biopsy
MH  - Carcinoma/*veterinary
MH  - *Chromosome Aberrations
MH  - *Comparative Genomic Hybridization
MH  - Computational Biology/methods
MH  - DNA Copy Number Variations
MH  - Dogs
MH  - Female
MH  - Genetic Loci
MH  - Genomics/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Urologic Neoplasms/*veterinary
PMC - PMC4501039
MID - NIHMS702432
EDAT- 2015/03/19 06:00
MHDA- 2016/02/04 06:00
PMCR- 2015/07/14
CRDT- 2015/03/19 06:00
PHST- 2015/01/10 00:00 [received]
PHST- 2015/02/10 00:00 [accepted]
PHST- 2015/02/07 00:00 [revised]
PHST- 2015/03/19 06:00 [entrez]
PHST- 2015/03/19 06:00 [pubmed]
PHST- 2016/02/04 06:00 [medline]
PHST- 2015/07/14 00:00 [pmc-release]
AID - 10.1007/s10577-015-9471-y [doi]
PST - ppublish
SO  - Chromosome Res. 2015 Jun;23(2):311-31. doi: 10.1007/s10577-015-9471-y. Epub 2015 
      Mar 18.