PMID- 25784450
OWN - NLM
STAT- MEDLINE
DCOM- 20160223
LR  - 20220317
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 84
IP  - 1
DP  - 2015 Jul 1
TI  - Regulation of embryonic development and apoptotic-related gene expression by 
      brain-derived neurotrophic factor in two different culture conditions in ovine.
PG  - 62-9
LID - S0093-691X(15)00085-0 [pii]
LID - 10.1016/j.theriogenology.2015.02.012 [doi]
AB  - In the present study, we aimed to evaluate effects of brain-derived neurotrophic 
      factor (BDNF) which is a member of neurotrophic factor family on developmental 
      competence of oocytes in sheep. In vitro maturation was performed in presence of 
      various concentrations (0, 10, and 100 ng/mL) of BDNF. Meiotic maturation, levels 
      of intracellular glutathione, embryonic developmental potential after 
      parthenogenetic activation, number of total and apoptotic cells in blastocysts, 
      and expression of Bax and Bcl-2 genes in blastocyst cells were determined. Under 
      unstressed condition, while at 100 ng/mL concentration, BDNF increased the IVM 
      rate; an increase of glutathione level was observed at 10 ng/mL concentration. 
      Moreover, when BDNF-treated oocytes were used for parthenogenetic activation, 
      more blastocyst at both 10 and 100 ng/mL levels was obtained in comparison with 
      the untreated group. Under heat stress (HS), the blastocyst rate was dramatically 
      reduced in untreated oocytes compared to that obtained from 10 ng/mL BDNF groups. 
      Total cell number in blastocysts was not affected by the treatment groups. The 
      mean of Terminal deoxynucleotidyl transferase dUTP nick end labeling 
      (TUNEL)-positive nuclei in blastocysts was not influenced by addition of BDNF in 
      medium and that presence or absence of thermal stress during IVM than the control 
      group. Moreover, our data revealed that the expression of Bax and Bcl-2 genes in 
      blastocysts was affected by both BDNF concentration and HS. Conclusively, 
      supplementation of IVM medium with 10 ng/mL BDNF had a beneficial effect on sheep 
      oocyte competence by increasing the rate of blastocyst especially when HS exists.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Abazari-Kia, Amir Hossein
AU  - Abazari-Kia AH
AD  - Department of Transgenic Animal Science, Stem Cell Technology Research Center, 
      Tehran, Iran.
FAU - Dehghani-Mohammadabadi, Maryam
AU  - Dehghani-Mohammadabadi M
AD  - Department of Transgenic Animal Science, Stem Cell Technology Research Center, 
      Tehran, Iran.
FAU - Mohammadi-Sangcheshmeh, Abdollah
AU  - Mohammadi-Sangcheshmeh A
AD  - Department of Animal and Poultry Science, College of Aburaihan, University of 
      Tehran, Pakdasht, Tehran, Iran.
FAU - Zhandi, Mahdi
AU  - Zhandi M
AD  - Department of Animal Science, College of Agriculture and Natural Resources, 
      University of Tehran, Karaj, Iran.
FAU - Salehi, Mohammad
AU  - Salehi M
AD  - Cellular and Molecular Biology Research Center, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran; Department of Biotechnology, School of Advanced 
      Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, 
      Iran. Electronic address: msalehi78@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150216
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Embryo Culture Techniques/*veterinary
MH  - Embryonic Development/*drug effects/genetics
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - In Vitro Oocyte Maturation Techniques/veterinary
MH  - Pregnancy
MH  - Sheep/*embryology/growth & development
OTO - NOTNLM
OT  - Apoptotic genes
OT  - Blastocyst
OT  - Brain-derived neurotrophic factor
OT  - Glutathione level
OT  - Heat stress
OT  - Sheep oocyte
EDAT- 2015/03/19 06:00
MHDA- 2016/02/26 06:00
CRDT- 2015/03/19 06:00
PHST- 2014/09/25 00:00 [received]
PHST- 2015/01/15 00:00 [revised]
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/03/19 06:00 [entrez]
PHST- 2015/03/19 06:00 [pubmed]
PHST- 2016/02/26 06:00 [medline]
AID - S0093-691X(15)00085-0 [pii]
AID - 10.1016/j.theriogenology.2015.02.012 [doi]
PST - ppublish
SO  - Theriogenology. 2015 Jul 1;84(1):62-9. doi: 10.1016/j.theriogenology.2015.02.012. 
      Epub 2015 Feb 16.