PMID- 25784985
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150318
LR  - 20201001
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 1
DP  - 2015
TI  - Functional characterization of OPN in human laryngeal squamous cell carcinoma and 
      its xenograft model in nude mice.
PG  - 164-72
AB  - BACKGROUND: Osteopontin (OPN) is involved in promotion of cancer cells by 
      regulating various facets of tumor progression such as cell proliferation, 
      angiogenesis and metastasis. To understand the role of OPN in laryngeal squamous 
      cell carcinoma (LSCC), we thus explored the biological function of OPN in LSCC 
      after silencing OPN expression by RNA interference (RNAi). METHOD: The OPN 
      expression in tumor tissues of LSCC was determined immunohistochemically in both 
      LSCC and adjacent normal tissues. Lentivirus vector with RNAi small hairpin gene 
      sequence of OPN (named LV-shOPN) was transfected into Hep-2 cells and 
      transplanted into BALB/c-nu mice. After siRNA transfection, the viability of 
      Hep-2 cells was examined by MTS, OPN expression was detected by Western blotting, 
      and tumor angiogenesis was assessed by microvessel densities (MVD). RESULTS: The 
      difference of positive rate of OPN in 72 cases LSCC (54 cases, 75.0%) and 
      adjacent normal tissues (15 cases, 20.8%) was statistically significant (P<0.001) 
      and the OPN expression was also significantly correlated with tumor stage, grade 
      and the presence of lymph node. Hep-2 cells infected with LV-shOPN significantly 
      decreased OPN expression, in comparison to cells with LV-shNon transfection (as 
      the control) (P<0.05). The constructed LV-shOPN effectively inhibited the 
      viability of Hep-2 cell and growth of xenograft tumors in nude mice (all 
      P<0.050). The expression of OPN and MVD was significantly decreased in xenograft 
      tumors (all P<0.05). CONCLUSION: RNAi silencing of OPN expression can 
      significantly inhibit tumor growth and angiogenesis of Hep-2 cells, and OPN may 
      be considered as one of gene targeting therapy for LSCC.
FAU - Chen, Jianqiu
AU  - Chen J
AD  - Department of Otolaryngology Head and Neck Surgery, General Hospital of Jinan 
      Military Region Jinan 250031, Shandong Province, PR China.
FAU - Zhou, Qi
AU  - Zhou Q
AD  - Department of Tumor Biological Treatment, The Third Affiliated Hospital, Soochow 
      University Changzhou 213003, Jiangsu Province, China.
FAU - Zhu, Chunsheng
AU  - Zhu C
AD  - Department of Otolaryngology Head and Neck Surgery, General Hospital of Jinan 
      Military Region Jinan 250031, Shandong Province, PR China.
FAU - Zhu, Minhui
AU  - Zhu M
AD  - Department of Otolaryngology Head and Neck Surgery, Changhai Hospital of The 
      Second Military Medical University Shanghai 200433, PR China.
FAU - Tian, Yongsheng
AU  - Tian Y
AD  - Department of Otolaryngology Head and Neck Surgery, Aerospace Center Hospital of 
      Beijing university Beijing 100049, PR China.
FAU - Li, Guojun
AU  - Li G
AD  - Department of Head and Neck Surgery, U.T. M.D. Anderson Cancer Center Houston, TX 
      77030, USA.
FAU - Tao, Xiaofeng
AU  - Tao X
AD  - Department of Radiology, Shanghai Ninth People's Hospital Affiliated Shanghai 
      Jiaotong University School of Medicine China.
FAU - Zheng, Hongliang
AU  - Zheng H
AD  - Department of Otolaryngology Head and Neck Surgery, Changhai Hospital of The 
      Second Military Medical University Shanghai 200433, PR China.
LA  - eng
PT  - Journal Article
DEP - 20150115
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4358440
OTO - NOTNLM
OT  - Osteopontin
OT  - RNA interference
OT  - laryngeal neoplasms
OT  - laryngeal squamous cell carcinoma
OT  - nude mice
EDAT- 2015/03/19 06:00
MHDA- 2015/03/19 06:01
PMCR- 2015/01/15
CRDT- 2015/03/19 06:00
PHST- 2014/11/12 00:00 [received]
PHST- 2015/01/14 00:00 [accepted]
PHST- 2015/03/19 06:00 [entrez]
PHST- 2015/03/19 06:00 [pubmed]
PHST- 2015/03/19 06:01 [medline]
PHST- 2015/01/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Jan 15;8(1):164-72. eCollection 2015.