PMID- 2578508
OWN - NLM
STAT- MEDLINE
DCOM- 19850320
LR  - 20081121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 134
IP  - 3
DP  - 1985 Mar
TI  - The use of haptenated-immunoglobulin molecules to induce tolerance in B cells 
      from neonatal mice.
PG  - 1436-41
AB  - The role of the Fc region of trinitrophenylated (TNP)-immunoglobulins (Ig) in 
      their ability to induce tolerance in immature B cells was examined. With the use 
      of B cells from neonatal mice, tolerogens that could or could not bind to Fc 
      receptors were assessed for their ability to induce tolerance. This was 
      accomplished by tolerizing spleen cells in bulk culture and assessing the degree 
      of tolerance by challenging the cells with the thymus-independent antigen 
      TNP-Brucella abortus (TNP-BA) in limiting dilution cultures. It was found that by 
      using tolerogens containing 10 to 11 haptens per Ig molecule, immature B cells 
      were very susceptible to tolerance induction. Mature B cells were not as 
      susceptible. This increased susceptibility was independent of the Fc portion of 
      the tolerogen, because TNP11-HGG and a TNP10-F(ab')2 induced equivalent degrees 
      of unresponsiveness. When the TNP density was lowered to approximately five 
      haptens per Ig molecule, those Ig molecules that contained Fc portions were 
      superior tolerogens with the use of B cells from 6-day-old mice. Thus, a 
      TNP4-HGG, TNP7-mouse IgG1, and TNP6-mouse IgG2a were more effective tolerogens 
      than either TNP5-F(ab')2 or TNP6-mouse IgG3. These results confirm previous 
      findings that immature B cells are inherently more susceptible to tolerance 
      induction than mature B cells. They also suggest that very lightly haptenated Ig 
      molecules may depend on Fc receptor-binding for effective tolerance induction. 
      Finally, by means of a cytofluorograph, the surface IgD (sIgD) and sIgM 
      phenotypes of splenic B cells from neonates of increasing age were determined. 
      When comparing the phenotype of maturing cells with their tolerance 
      susceptibilities, a correlation between the appearance of sIgD and the 
      acquisition of resistance to tolerance was observed.
FAU - Waldschmidt, T J
AU  - Waldschmidt TJ
FAU - Vitetta, E S
AU  - Vitetta ES
LA  - eng
GR  - AI-11851/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes)
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Myeloma Proteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Fc)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Animals, Newborn/*immunology
MH  - B-Lymphocytes/*immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Epitopes/immunology
MH  - Female
MH  - Haptens/analysis/*immunology
MH  - Humans
MH  - *Immune Tolerance
MH  - Immunity, Innate
MH  - Immunoglobulins/metabolism/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Myeloma Proteins/metabolism
MH  - Phenotype
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Receptors, Antigen, B-Cell/classification
MH  - Receptors, Fc/analysis/physiology
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1985 Mar;134(3):1436-41.