PMID- 25786325
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20191210
IS  - 1029-2470 (Electronic)
IS  - 1029-2470 (Linking)
VI  - 49
IP  - 8
DP  - 2015
TI  - Effect of elite physical exercise by triathletes on seven catabolites of DNA 
      oxidation.
PG  - 973-83
LID - 10.3109/10715762.2015.1025388 [doi]
AB  - The oxidized nucleoside 8-hydroxy-2'-deoxyguanosine has been widely studied as a 
      marker of DNA oxidation; however, data on the occurrence of other metabolites in 
      plasma that are related to DNA damage are scarce. We have applied an improved, 
      sensitive, robust, and reliable method, involving solid phase extraction and 
      ultrahigh-performance liquid chromatography (UHPLC)-tandem mass spectrometry 
      (MS/MS), to the precise quantitation of seven metabolites in the plasma of 15 
      elite triathletes after a 2-week training program. All compounds were eluted in 
      the first 1.6 min, with limits of detection and quantification ranging between 
      0.001 and 0.3 ng.mL(-1) and 0.009 and 0.6 ng.mL(-1), respectively. Four compounds 
      were detected in plasma: guanosine-3'-5'-cyclic monophosphate, 8-hydroxyguanine, 
      8-hydroxy-2'-deoxyguanosine, and 8-nitroguanosine. After two weeks of training, 
      8-hydroxyguanine exhibited the highest increase (from 0.031 +/- 0.008 nM to 0.036 +/- 
      0.012 nM) (p < 0.05), which could be related to the enhanced activity of 
      DNA-repairing enzymes that excise this oxidized base. Increased levels of 
      guanosine-3'-5'-cyclic monophosphate and 8-hydroxy-2'-deoxyguanosine were also 
      observed. In contrast, levels of 8-nitroguanosine (p < 0.05) were significantly 
      reduced, which might be a protective measure as this compound strongly stimulates 
      the generation of superoxide radicals, and its excess is related to pathologies 
      such as microbial (viral) infections and other inflammatory and degenerative 
      disorders. The results obtained indicate an induced adaptive response to the 
      increased oxidative stress related to elite training, and point to the benefits 
      associated with regular exercise.
FAU - Villano, D
AU  - Villano D
AD  - Department of Food Science and Technology, CEBAS-CSIC , Murcia , Spain.
FAU - Vilaplana, C
AU  - Vilaplana C
FAU - Medina, S
AU  - Medina S
FAU - Cejuela-Anta, R
AU  - Cejuela-Anta R
FAU - Martinez-Sanz, J M
AU  - Martinez-Sanz JM
FAU - Gil, P
AU  - Gil P
FAU - Genieser, H-G
AU  - Genieser HG
FAU - Ferreres, F
AU  - Ferreres F
FAU - Gil-Izquierdo, A
AU  - Gil-Izquierdo A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150408
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (8-nitroguanine)
RN  - 0 (8-nitroguanosine)
RN  - 0 (Nitro Compounds)
RN  - 12133JR80S (Guanosine)
RN  - 3868-31-3 (8-hydroxyguanosine)
RN  - 5614-64-2 (8-hydroxyguanine)
RN  - 5Z93L87A1R (Guanine)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - 9007-49-2 (DNA)
RN  - G9481N71RO (Deoxyguanosine)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - *Athletes
MH  - Cyclic GMP/blood
MH  - DNA/*blood
MH  - DNA Fragmentation
MH  - Deoxyguanosine/analogs & derivatives/blood
MH  - Female
MH  - Guanine/analogs & derivatives/blood
MH  - Guanosine/analogs & derivatives/blood
MH  - Humans
MH  - Limit of Detection
MH  - Male
MH  - Nitro Compounds/blood
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Physical Conditioning, Human
MH  - Young Adult
OTO - NOTNLM
OT  - 8-hydroxy-deoxyguanosine
OT  - 8-hydroxyguanine
OT  - DNA oxidation biomarkers
OT  - oxidative stress
OT  - sport
EDAT- 2015/03/20 06:00
MHDA- 2016/04/19 06:00
CRDT- 2015/03/20 06:00
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
AID - 10.3109/10715762.2015.1025388 [doi]
PST - ppublish
SO  - Free Radic Res. 2015;49(8):973-83. doi: 10.3109/10715762.2015.1025388. Epub 2015 
      Apr 8.