PMID- 25786490
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20170406
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 27
IP  - 6
DP  - 2015 Jul
TI  - Effects of aging on gene expression and mitochondrial DNA in the equine oocyte 
      and follicle cells.
PG  - 925-33
LID - 10.1071/RD14472 [doi]
AB  - We hypothesised that advanced mare age is associated with follicle and oocyte 
      gene alterations. The aims of the study were to examine quantitative and temporal 
      differences in mRNA for LH receptor (LHR), amphiregulin (AREG) and epiregulin 
      (EREG) in granulosa cells, phosphodiesterase (PDE) 4D in cumulus cells and PDE3A, 
      G-protein-coupled receptor 3 (GPR3), growth differentiation factor 9 (GDF9), bone 
      morphogenetic protein 15 (BMP15) and mitochondrial (mt) DNA in oocytes. Samples 
      were collected from dominant follicles of Young (3-12 years) and Old (>/=20 years) 
      mares at 0, 6, 9 and 12h after administration of equine recombinant LH. LHR mRNA 
      declined after 0h in Young mares, with no time effect in Old mares. For both 
      ages, gene expression of AREG was elevated at 6 and 9h and EREG was expression 
      was elevated at 9h, with higher expression in Old than Young mares. Cumulus cell 
      PDE4D expression increased by 6h (Old) and 12h (Young). Oocyte GPR3 expression 
      peaked at 9 and 12h in Young and Old mares, respectively. Expression of PDE3A 
      increased at 6h, with the increase greater in oocytes from Old than Young mares 
      at 6 and 9h. Mean GDF9 and BMP15 transcripts were higher in Young than Old, with 
      a peak at 6h. Copy numbers of mtDNA did not vary over time in oocytes from Young 
      mares, but a temporal decrease was observed in oocytes from Old mares. The 
      results support an age-associated asynchrony in the expression of genes that are 
      essential for follicular and oocyte maturation before ovulation.
FAU - Campos-Chillon, Fernando
AU  - Campos-Chillon F
AD  - California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, CA 
      93407, USA.
FAU - Farmerie, Todd A
AU  - Farmerie TA
AD  - Washington State University, PO Box 647520, Pullman, WA 99164, USA.
FAU - Bouma, Gerrit J
AU  - Bouma GJ
AD  - Colorado State University, 1693 Campus Delivery, Fort Collins, CO 80523, USA.
FAU - Clay, Colin M
AU  - Clay CM
AD  - Colorado State University, 1693 Campus Delivery, Fort Collins, CO 80523, USA.
FAU - Carnevale, Elaine M
AU  - Carnevale EM
AD  - Colorado State University, 1693 Campus Delivery, Fort Collins, CO 80523, USA.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Amphiregulin)
RN  - 0 (Bone Morphogenetic Protein 15)
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (Epiregulin)
RN  - 0 (Growth Differentiation Factor 9)
RN  - 0 (Receptors, LH)
RN  - EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 4)
SB  - IM
MH  - Aging/genetics/*metabolism
MH  - Amphiregulin/genetics/metabolism
MH  - Animals
MH  - Bone Morphogenetic Protein 15/genetics/metabolism
MH  - Cumulus Cells/*metabolism
MH  - Cyclic Nucleotide Phosphodiesterases, Type 4/genetics/metabolism
MH  - DNA, Mitochondrial/genetics/*metabolism
MH  - Epiregulin/genetics/metabolism
MH  - Female
MH  - *Gene Expression
MH  - Growth Differentiation Factor 9/genetics/metabolism
MH  - Horses
MH  - Oocytes/*metabolism
MH  - Ovarian Follicle/*metabolism
MH  - Receptors, LH/genetics/metabolism
MH  - Transcriptome
EDAT- 2015/03/20 06:00
MHDA- 2017/04/07 06:00
CRDT- 2015/03/20 06:00
PHST- 2014/11/26 00:00 [received]
PHST- 2015/02/18 00:00 [accepted]
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - RD14472 [pii]
AID - 10.1071/RD14472 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2015 Jul;27(6):925-33. doi: 10.1071/RD14472.