PMID- 25786520
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20161125
IS  - 1880-3989 (Electronic)
IS  - 0388-1350 (Linking)
VI  - 40
IP  - 2
DP  - 2015 Apr
TI  - Further development of LLNA:DAE method as stand-alone skin-sensitization testing 
      method and applied for evaluation of relative skin-sensitizing potency between 
      chemicals.
PG  - 137-50
LID - 10.2131/jts.40.137 [doi]
AB  - To date, there has been no well-established local lymph node assay (LLNA) that 
      includes an elicitation phase. Therefore, we developed a modified local lymph 
      node assay with an elicitation phase (LLNA:DAE) to discriminate true skin 
      sensitizers from chemicals that gave borderline positive results and previously 
      reported this assay. To develop the LLNA:DAE method as a useful stand-alone 
      testing method, we investigated the complete procedure for the LLNA:DAE method 
      using hexyl cinnamic aldehyde (HCA), isoeugenol, and 2,4-dinitrochlorobenzene 
      (DNCB) as test compounds. We defined the LLNA:DAE procedure as follows: in the 
      dose-finding test, four concentrations of chemical applied to dorsum of the right 
      ear on days 1, 2, and 3 and dorsum of both ears on day 10. Ear thickness and skin 
      irritation score were measured on days 1, 3, 5, 10, and 12. Local lymph nodes 
      were excised and weighed on day 12. The test dose for the primary LLNA:DAE study 
      was selected as the dose that gave the highest left ear lymph node weight in the 
      dose-finding study, or the lowest dose that produced a left ear lymph node of 
      over 4 mg. This procedure was validated using nine different chemicals. 
      Furthermore, qualitative relationship was observed between the degree of 
      elicitation response in the left ear lymph node and the skin sensitizing potency 
      of 32 chemicals tested in this study and the previous study. These results 
      indicated that LLNA:DAE method was as first LLNA method that was able to evaluate 
      the skin sensitizing potential and potency in elicitation response.
FAU - Yamashita, Kunihiko
AU  - Yamashita K
AD  - Corporate Research Center, Daicel Corporation, 1239 Shinzaike, Aboshi-ku, Himeji, 
      Hyogo, 671-1283, Japan; Faculty of Engineering, Dept. of Materials Science and 
      Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogoya, Yokohama, 
      Kanagawa, 240-8501, Japan.
FAU - Shinoda, Shinsuke
AU  - Shinoda S
FAU - Hagiwara, Saori
AU  - Hagiwara S
FAU - Itagaki, Hiroshi
AU  - Itagaki H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Toxicol Sci
JT  - The Journal of toxicological sciences
JID - 7805798
RN  - 0 (Nitrobenzoates)
RN  - 101-86-0 (hexyl cinnamic aldehyde)
RN  - 3T8H1794QW (Eugenol)
RN  - 5M0MWY797U (isoeugenol)
RN  - 610-30-0 (2,4-dinitrobenzoic acid)
RN  - 7864XYD3JJ (Acrolein)
SB  - IM
MH  - Acrolein/adverse effects/*analogs & derivatives
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Ear
MH  - Eugenol/adverse effects/*analogs & derivatives
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/*immunology
MH  - Mice, Inbred CBA
MH  - Nitrobenzoates/*adverse effects
MH  - Skin Irritancy Tests/*methods
EDAT- 2015/03/20 06:00
MHDA- 2015/10/27 06:00
CRDT- 2015/03/20 06:00
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - 10.2131/jts.40.137 [doi]
PST - ppublish
SO  - J Toxicol Sci. 2015 Apr;40(2):137-50. doi: 10.2131/jts.40.137.