PMID- 25787001
OWN - NLM
STAT- MEDLINE
DCOM- 20150508
LR  - 20230711
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 37
IP  - 3
DP  - 2015 Mar
TI  - [Correlation between type 2 diabetes and DNA methylation and mRNA expression of 
      APN in abdominal adipose tissues in Xinjiang Uygur population].
PG  - 269-275
LID - 10.16288/j.yczz.14-199 [doi]
AB  - To investigate the relationship between type 2 diabetes mellitus (T2DM) onset and 
      development and mRNA expression and promoter methylation of adiponectin (APN) 
      gene in abdominal adipose tissues of Xinjiang Uygur population, abdominal adipose 
      tissues of omentum were collected and divided into control, obesity and T2DM 
      groups. The status of APN promoter methylation was detected by denaturing high 
      performance liquid chromatography (DHPLC), while the mRNA expression level of APN 
      was detected by RT-PCR. Results show that methylation positive rate of APN was at 
      the lowest level in control, middel in obesity and highest in T2DM groups, and 
      the differences are statistically significant. Comparing the APN mRNA relative 
      copy number of adipose tissue in each group, we found that the relative copy 
      number of APN in control group is significantly higher than that of obesity and 
      T2DM groups. There is a negative correlation between the mRNA expression level of 
      APN in abdominal adipose tissue and fasting plasma glucose (FPG), glycosylated 
      hemoglobin (HbA1c) and triglyceride (TG) level. There is a negative correlation 
      in DNA promoter methylation and mRNA expression of APN gene. Relative copy number 
      of APN in DNA methylation positive group is significantly lower than that of the 
      negative group. In conclusion, increased APN promoter methylation results in 
      decreased mRNA expression, which induces glucose and lipid metabolic disorder, 
      thus contributing to the initiation and development of T2DM in Xinjiang Uygur 
      population.
FAU - Zhang, Jun
AU  - Zhang J
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Zhang, Wang-Qiang
AU  - Zhang WQ
AD  - Department of Cardiology, First Affiliated Hospital, Medicine School of Shihezi 
      University, Shihezi 832000,China.
FAU - Ding, Yu-Lei
AU  - Ding YL
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Xu, Peng
AU  - Xu P
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Wang, Ting-Ting
AU  - Wang TT
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Xu, Wen-Jing
AU  - Xu WJ
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Lu, Huan
AU  - Lu H
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Liu, Zong-Zhi
AU  - Liu ZZ
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
FAU - Xie, Jian-Xin
AU  - Xie JX
AD  - The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education 
      , Medicine School of Shihezi University, Shihezi 832000,China.
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
RN  - 0 (Adiponectin)
RN  - 0 (Blood Glucose)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Abdominal Fat/*metabolism
MH  - Adiponectin/*genetics/metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blood Glucose/metabolism
MH  - China/ethnology
MH  - *DNA Methylation
MH  - Diabetes Mellitus, Type 2/ethnology/*genetics/metabolism
MH  - Fasting
MH  - Female
MH  - Gene Dosage
MH  - Glycated Hemoglobin
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Obesity/ethnology/genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Young Adult
EDAT- 2015/03/20 06:00
MHDA- 2015/05/09 06:00
CRDT- 2015/03/20 06:00
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2015/05/09 06:00 [medline]
AID - 2015-3-269 [pii]
AID - 10.16288/j.yczz.14-199 [doi]
PST - ppublish
SO  - Yi Chuan. 2015 Mar;37(3):269-275. doi: 10.16288/j.yczz.14-199.