PMID- 25787735
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20181113
IS  - 2190-3948 (Electronic)
IS  - 2190-393X (Linking)
VI  - 5
IP  - 2
DP  - 2015 Apr
TI  - Nanofiber-mediated release of retinoic acid and brain-derived neurotrophic factor 
      for enhanced neuronal differentiation of neural progenitor cells.
PG  - 89-100
LID - 10.1007/s13346-013-0131-5 [doi]
AB  - The treatment of an injured central nervous system using stem-cell-based 
      regenerative medicine still faces considerable hurdles that need to be overcome. 
      Chief among which is the lack of efficient strategies to generate functional 
      neurons from stem cells. The sustained delivery of biochemical cues and 
      synergistic topographical signaling from electrospun nanofibrous scaffolds may be 
      a potential strategy to enhance neuronal differentiation of stem cells for 
      therapeutic purposes. In this study, retinoic acid (RA) and brain-derived 
      neurotrophic factor (BDNF) were encapsulated into a copolymer of epsilon-caprolactone 
      and ethyl ethylene phosphate to form a multifunctional, electrospun nanofibrous 
      scaffold. Sustained release of RA and BDNF was achieved for at least 7 and 14 
      days, respectively. Despite lower cumulative release of drugs as compared to 
      bolus delivery to plain nanofibers (at least 2x and 50x lower for RA and BDNF, 
      respectively), nanofiber-mediated delivery of RA and/or BDNF resulted in similar 
      capacity for neuronal differentiation of mouse neural progenitor cells (NPCs). In 
      addition, nanofiber topography significantly increased neuronal differentiation 
      (with BDNF, 47.4% Map2(+) cells on 2D vs. 53.4 to 56.5% on nanofibers, p < 0.05) 
      and reduced glial cell differentiation. BDNF was a more potent inducer of 
      neuronal differentiation than RA. RA supplementation alone resulted in minimal 
      effect on NPC differentiation, and dual supplementation of RA and BDNF did not 
      further enhance the neuronal differentiation of NPCs. Collectively, the results 
      suggest that synergistic effects of nanofiber topography and sustained delivery 
      of RA and/or BDNF may contribute towards the design of a multifunctional 
      artificial stem cell niche for NPC neuronal differentiation.
FAU - Low, Wei Ching
AU  - Low WC
AD  - Division of Chemical and Biomolecular Engineering, School of Chemical and 
      Biomedical Engineering, Nanyang Technological University, Nanyang, 637459, 
      Singapore.
FAU - Rujitanaroj, Pim-On
AU  - Rujitanaroj PO
FAU - Wang, Feng
AU  - Wang F
FAU - Wang, Jun
AU  - Wang J
FAU - Chew, Sing Yian
AU  - Chew SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Drug Deliv Transl Res
JT  - Drug delivery and translational research
JID - 101540061
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Caproates)
RN  - 0 (Lactones)
RN  - 5688UTC01R (Tretinoin)
RN  - 56RE988L1R (caprolactone)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Caproates/chemistry
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Lactones/chemistry
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Scanning
MH  - Nanofibers/*administration & dosage/ultrastructure
MH  - Neural Stem Cells/cytology/*drug effects
MH  - Neurons/cytology
MH  - Tretinoin/*administration & dosage
EDAT- 2015/03/20 06:00
MHDA- 2016/01/05 06:00
CRDT- 2015/03/20 06:00
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
AID - 10.1007/s13346-013-0131-5 [doi]
PST - ppublish
SO  - Drug Deliv Transl Res. 2015 Apr;5(2):89-100. doi: 10.1007/s13346-013-0131-5.