PMID- 25788442
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20170428
IS  - 1744-7917 (Electronic)
IS  - 1672-9609 (Linking)
VI  - 23
IP  - 5
DP  - 2016 Oct
TI  - Molecular characterization and diversity analysis of bacterial communities 
      associated with Dialeurolonga malleswaramensis (Hemiptera: Aleyrodidae) adults 
      using 16S rDNA amplicon pyrosequencing and FISH.
PG  - 704-11
LID - 10.1111/1744-7917.12220 [doi]
AB  - Dialeurolonga malleswaramensis Sundararaj (Hemiptera: Aleyrodidae) is a 
      phytophagous sap sucking insect. It infests Polyalthia longifolia, an important 
      avenue tree of India, effective in alleviating noise pollution and having immense 
      medicinal importance. Samples of this insect were collected from Polyalthia 
      longifolia. The cytochrome c oxidase subunit I gene (mtCO1) helped in the 
      molecular characterization of the insect. This study reports the bacterial 
      diversity in D. malleswaramensis adults by high throughput 16S rDNA amplicon 
      pyrosequencing. The major genera identified were Portiera and Arsenophonus. Other 
      bacterial genera detected were uncultured alpha proteobacterium, Sphingopyxis and 
      Methylobacterium. We also employed fluorescence in situ hybridization (FISH) in 
      whole mount samples to confirm the presence of dominant endosymbionts Portiera 
      and Arsenophonus to the bacteriocyte of D. malleswaramensis. This study concludes 
      that combining techniques like 16S rDNA amplicon pyrosequencing and FISH reveal 
      both dominant and rare bacteria. The data also predict the evolutionary position 
      of this pest with respect to other whitefly species using a mitochondrial marker.
CI  - (c) 2015 Institute of Zoology, Chinese Academy of Sciences.
FAU - Pandey, Neeti
AU  - Pandey N
AD  - Gut Biology Lab, Room No 117, Department of Zoology, University of Delhi, Delhi, 
      110007, India.
FAU - Rajagopal, Raman
AU  - Rajagopal R
AD  - Gut Biology Lab, Room No 117, Department of Zoology, University of Delhi, Delhi, 
      110007, India. zoorajagopal@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20150519
PL  - Australia
TA  - Insect Sci
JT  - Insect science
JID - 101266965
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*genetics/isolation & purification
MH  - DNA, Bacterial/*genetics
MH  - Hemiptera/genetics/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - India
MH  - Mitochondria/genetics
MH  - Phylogeny
MH  - Polyalthia/parasitology
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Symbiosis
OTO - NOTNLM
OT  - 16S rDNA amplicon pyrosequencing
OT  - Dialeurolonga malleswaramensis
OT  - FISH
OT  - bacterial diversity
OT  - mtCO1
EDAT- 2015/03/20 06:00
MHDA- 2017/04/30 06:00
CRDT- 2015/03/20 06:00
PHST- 2014/02/15 00:00 [accepted]
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
AID - 10.1111/1744-7917.12220 [doi]
PST - ppublish
SO  - Insect Sci. 2016 Oct;23(5):704-11. doi: 10.1111/1744-7917.12220. Epub 2015 May 
      19.