PMID- 25788649
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20150617
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 56
IP  - 4
DP  - 2015 Apr
TI  - In Vivo Imaging of Subretinal Bleb-Induced Outer Retinal Degeneration in the 
      Rabbit.
PG  - 2423-30
LID - 10.1167/iovs.14-16208 [doi]
AB  - PURPOSE: To analyze the morphologic effects of subretinal blebs in rabbits using 
      real-time imaging by spectral-domain optical coherence tomography (SD-OCT), 
      infrared-confocal scanning laser ophthalmoscopy (IR-cSLO), and blue-light fundus 
      autofluorescence (BAF). METHODS: Subretinal blebs of PBS or balanced salt 
      solution (BSS) were induced in albino or pigmented rabbits using a transvitreal 
      pars plana technique. Spectral-domain optical coherence tomography, IR-cSLO, and 
      BAF were done at multiple intervals for up to 12 weeks after subretinal bleb 
      injection. The morphologic effects were compared with histologic analysis on 
      hematoxylin-eosin-stained sections of the neurosensory retina and on flat-mounts 
      of phalloidin-labeled RPE. RESULTS: Scans of SD-OCT of the normal rabbit 
      posterior segment revealed 11 bands including six layers of the photoreceptors. 
      Subretinal blebs of PBS or BSS caused acute swelling of the neurosensory retina 
      followed by gradual atrophy. Outer retinal thickness was significantly reduced 
      with pronounced degeneration of all the photoreceptor OCT layers. En face IR-cSLO 
      showed a hyperreflective area corresponding to the progressive photoreceptor 
      degeneration, whereas BAF revealed both hyper- and hypofluorescent changes in the 
      RPE layer. The in vivo results were confirmed by histology and on subretinal 
      flatmounts demonstrating extensive photoreceptor loss and disruption of the RPE 
      mosaic. CONCLUSIONS: Subretinal blebs induce pronounced photoreceptor 
      degeneration and RPE changes in the rabbit as demonstrated by in vivo imaging 
      using SD-OCT, IR-cSLO, and BAF.
FAU - Bartuma, Hammurabi
AU  - Bartuma H
FAU - Petrus-Reurer, Sandra
AU  - Petrus-Reurer S
FAU - Aronsson, Monica
AU  - Aronsson M
FAU - Westman, Sofie
AU  - Westman S
FAU - Andre, Helder
AU  - Andre H
FAU - Kvanta, Anders
AU  - Kvanta A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - *Diagnostic Imaging
MH  - Disease Models, Animal
MH  - Fluorescein Angiography/*methods
MH  - Fundus Oculi
MH  - Ophthalmoscopy/*methods
MH  - Photoreceptor Cells, Vertebrate/*pathology
MH  - Posterior Eye Segment/*pathology
MH  - Rabbits
MH  - Reproducibility of Results
MH  - Retinal Degeneration/*diagnosis/etiology
MH  - Tomography, Optical Coherence/*methods
EDAT- 2015/03/20 06:00
MHDA- 2015/08/26 06:00
CRDT- 2015/03/20 06:00
PHST- 2015/03/20 06:00 [entrez]
PHST- 2015/03/20 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
AID - iovs.14-16208 [pii]
AID - 10.1167/iovs.14-16208 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2015 Apr;56(4):2423-30. doi: 10.1167/iovs.14-16208.