PMID- 2578914
OWN - NLM
STAT- MEDLINE
DCOM- 19850412
LR  - 20190820
IS  - 0143-5221 (Print)
IS  - 0143-5221 (Linking)
VI  - 68
IP  - 4
DP  - 1985 Apr
TI  - Human mast cells recovered by bronchoalveolar lavage: their morphology, histamine 
      release and the effects of sodium cromoglycate.
PG  - 427-32
AB  - Mast cells make up between 0.5 and 3% (mean 1.35%) of total cells recovered by 
      bronchoalveolar lavage (BAL). The majority of these cells have the morphological 
      characteristics of mucosal mast cells in that they fail to stain in the alcian 
      blue-safranin reaction after fixation in formol-saline but stain well after 
      fixation in Carnoy's solution. Cells staining with berberine sulphate were seen 
      in only four of the 26 lavages. BAL cells released histamine in response to 
      anti-human immunoglobulin E (IgE) in a dose-dependent manner that was optimal at 
      a dilution of anti-IgE of 1:100. Maximum release was obtained by 2 min. Histamine 
      release was completely inhibited by a combination of 2-deoxyglucose (5 mmol/l) 
      and antimycin A (1 mumol/l). Disodium cromoglycate (DSCG) significantly inhibited 
      this histamine release at 1 mmol/l (P less than 0.02), 100 mumol/l (P less than 
      0.002) and 10 mumol/l (P less than 0.003), with maximum inhibition of 50.1% at 10 
      mumol/l.
FAU - Flint, K C
AU  - Flint KC
FAU - Leung, K B
AU  - Leung KB
FAU - Pearce, F L
AU  - Pearce FL
FAU - Hudspith, B N
AU  - Hudspith BN
FAU - Brostoff, J
AU  - Brostoff J
FAU - Johnson, N M
AU  - Johnson NM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - Q2WXR1I0PK (Cromolyn Sodium)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antibodies, Anti-Idiotypic/immunology
MH  - Bronchi/cytology
MH  - Cell Count
MH  - Cromolyn Sodium/*pharmacology
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Histamine Release/*drug effects
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Male
MH  - Mast Cells/*cytology/metabolism
MH  - Middle Aged
MH  - Pulmonary Alveoli/cytology
MH  - Therapeutic Irrigation
EDAT- 1985/04/01 00:00
MHDA- 1985/04/01 00:01
CRDT- 1985/04/01 00:00
PHST- 1985/04/01 00:00 [pubmed]
PHST- 1985/04/01 00:01 [medline]
PHST- 1985/04/01 00:00 [entrez]
AID - 10.1042/cs0680427 [doi]
PST - ppublish
SO  - Clin Sci (Lond). 1985 Apr;68(4):427-32. doi: 10.1042/cs0680427.