PMID- 25792371 OWN - NLM STAT- MEDLINE DCOM- 20160303 LR - 20150613 IS - 1523-6536 (Electronic) IS - 1083-8791 (Linking) VI - 21 IP - 7 DP - 2015 Jul TI - Low Counts of Plasmacytoid Dendritic Cells after Engraftment Are Associated with High Early Mortality after Allogeneic Stem Cell Transplantation. PG - 1223-9 LID - S1083-8791(15)00182-2 [pii] LID - 10.1016/j.bbmt.2015.03.010 [doi] AB - Dendritic cells (DCs) are antigen-presenting cells that drive immune responses and tolerance and are divided in different subsets: myeloid DCs (mDCs: lineage-; HLA-DR+, 11c+), plasmacytoid dendritic cells (pDCs: HLA-DR+, CD123+), and monocyte-derived DCs (moDC: lineage-, 11c+, 16+). After hematopoietic stem cell transplantation (HSCT), low DC counts in the recipients' peripheral blood (PB) have been associated with worse outcomes, but the relevance of DC graft content remains unclear, and there are few data in the setting of unrelated donor HSCT. We evaluated the DC graft content and monitored DC recovery in PB from 111 HSCT recipients (median age, 17 years; range 1 to 74), who received bone marrow (46%), umbilical cord blood (32%), or PB (22%) from unrelated (81%) or related donors (19%). In 86 patients with sustained allogeneic recovery, patients with higher counts of all DC subsets (pDC, mDC, and moDC) 3 weeks after engraftment had lower incidence of nonrelapse mortality (NMR) and acute graft-versus-host disease (aGVHD) and better survival. pDC counts were associated with more striking results: patients with higher pDC counts had much lower incidences of NRM (3% versus 47%, P < .0001), lower incidence of aGVHD (24% versus 67%, P < .0001), and better overall survival (92% versus 45%, P < .0001). In contrast, higher pDC counts in the graft was associated with an increased risk of aGVHD (55% versus 26%, P = .02). Our results indicate that DC counts are closely correlated with HSCT outcomes and warrant further prospective evaluation and possible early therapeutic interventions to ameliorate severe aGVHD and decrease mortality. CI - Copyright (c) 2015 American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved. FAU - Goncalves, Matheus Vescovi AU - Goncalves MV AD - Disciplina de Hematologia e Hemoterapia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. Electronic address: matheus.vescovi@gmail.com. FAU - Yamamoto, Mihoko AU - Yamamoto M AD - Disciplina de Hematologia e Hemoterapia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Kimura, Eliza Yurico Sugano AU - Kimura EY AD - Disciplina de Hematologia e Hemoterapia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Colturato, Vergilio Antonio Rensi AU - Colturato VA AD - Fundacao Dr Amaral Carvalho, Jau, Brazil. FAU - de Souza, Mair Pedro AU - de Souza MP AD - Fundacao Dr Amaral Carvalho, Jau, Brazil. FAU - Mauad, Marcos AU - Mauad M AD - Fundacao Dr Amaral Carvalho, Jau, Brazil. FAU - Ikoma, Maura Valerio AU - Ikoma MV AD - Fundacao Dr Amaral Carvalho, Jau, Brazil. FAU - Novis, Yana AU - Novis Y AD - Centro de Oncologia, Hospital Sirio Libanes, Sao Paulo, Brazil. FAU - Rocha, Vanderson AU - Rocha V AD - Eurocord/Saint-Louis Hospital, Paris, France; Churchill Hospital, Oxford University Hospital, Oxford, United Kingdom. FAU - Ginani, Valeria Cortez AU - Ginani VC AD - Instituto de Oncologia Pediatrica, Sao Paulo, Brazil. FAU - Wanderley de Oliveira Felix, Olga Margareth AU - Wanderley de Oliveira Felix OM AD - Instituto de Oncologia Pediatrica, Sao Paulo, Brazil. FAU - Seber, Adriana AU - Seber A AD - Hospital Samaritano, Sao Paulo, Brazil. FAU - Kerbauy, Fabio Rodrigues AU - Kerbauy FR AD - Disciplina de Hematologia e Hemoterapia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil; Hospital Israelita Albert Einstein, Sao Paulo, Brazil. FAU - Hamerschlak, Nelson AU - Hamerschlak N AD - Hospital Israelita Albert Einstein, Sao Paulo, Brazil. FAU - Orfao, Alberto AU - Orfao A AD - Centro de Investigacion del Cancer (CIC,I BMCC USAL-CSIC); Servicio General de Citometria, Departamento de Medicina, IBSAL, Universidad de Salamanca, Salamanca, Spain. FAU - Rodrigues, Celso Arrais AU - Rodrigues CA AD - Disciplina de Hematologia e Hemoterapia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil; Centro de Oncologia, Hospital Sirio Libanes, Sao Paulo, Brazil. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150317 PL - United States TA - Biol Blood Marrow Transplant JT - Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation JID - 9600628 RN - 0 (Immunosuppressive Agents) RN - 0 (Myeloablative Agonists) SB - IM MH - Acute Disease MH - Adolescent MH - Adult MH - Aged MH - Bone Marrow Transplantation/*adverse effects MH - Cell Count MH - Cell Lineage/immunology MH - Child MH - Child, Preschool MH - Cord Blood Stem Cell Transplantation/*adverse effects MH - Dendritic Cells/classification/immunology/*pathology MH - Female MH - Graft vs Host Disease/drug therapy/etiology/immunology/*mortality MH - Hematologic Neoplasms/immunology/*mortality/pathology/therapy MH - Hematopoietic Stem Cell Transplantation/*adverse effects MH - Humans MH - Immunosuppressive Agents/therapeutic use MH - Infant MH - Male MH - Middle Aged MH - Myeloablative Agonists/therapeutic use MH - Siblings MH - Survival Analysis MH - *Transplantation Conditioning MH - Transplantation, Homologous MH - Unrelated Donors OTO - NOTNLM OT - Dendritic cells OT - Graft-versus-host disease OT - Immune recovery OT - Nonrelapse mortality OT - Transplantation EDAT- 2015/03/21 06:00 MHDA- 2016/03/05 06:00 CRDT- 2015/03/21 06:00 PHST- 2014/12/01 00:00 [received] PHST- 2015/03/11 00:00 [accepted] PHST- 2015/03/21 06:00 [entrez] PHST- 2015/03/21 06:00 [pubmed] PHST- 2016/03/05 06:00 [medline] AID - S1083-8791(15)00182-2 [pii] AID - 10.1016/j.bbmt.2015.03.010 [doi] PST - ppublish SO - Biol Blood Marrow Transplant. 2015 Jul;21(7):1223-9. doi: 10.1016/j.bbmt.2015.03.010. Epub 2015 Mar 17.