PMID- 25795219
OWN - NLM
STAT- MEDLINE
DCOM- 20160128
LR  - 20171116
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 44
IP  - 2
DP  - 2015 Jun
TI  - Molecular characterization, genomic structure and expressional profiles of a CXC 
      chemokine receptor 4 (CXCR4) from rock bream Oplegnathus fasciatus.
PG  - 471-7
LID - S1050-4648(15)00111-4 [pii]
LID - 10.1016/j.fsi.2015.03.012 [doi]
AB  - The CXC chemokine receptor 4 (CXCR4) is the cognate receptor of the CXC chemokine 
      ligand 12 (CXCL12) and plays a pivotal role under immune-pathophysiological 
      conditions. In the current study, the CXCR4 homolog of Oplegnathus fasciatus 
      (OfCXCR4) was sequenced and the mRNA expression levels were characterized. The 
      genomic structure of the cloned OfCXCR4 coding region (2094 bp) revealed a 
      bi-exonic element, where the open reading frame (ORF) appears split by a single 
      intron. Analysis of the ORF (1134 bp) of OfCXCR4 revealed a predicted protein of 
      42.1 kDa with typical seven transmembrane (TM) domain architecture and several 
      conserved structural features, including two cysteine residues forming a 
      predicted disulfide bond, a characteristic CXC motif (containing CYC) and a 
      G-protein-coupled receptor (GPCR) family 1 signature. Furthermore, based on 
      comparative analysis, the structure OfCXCR4 appears well conserved at both the 
      genomic DNA and the amino acid levels. Phylogenic analysis of OfCXCR4 revealed 
      that the greatest homology was with its teleostean relatives. Expression studies 
      showed ubiquitous OfCXCR4 transcription, mainly in immune organs, with the 
      highest levels in the head kidney. Examination of OfCXCR4 transcriptional 
      regulation post injection to different stimuli or pathogens revealed a 
      significant modulation of mRNA expression as detected by reverse 
      transcription-quantitative real-time PCR. Evidence of various transcription 
      factor binding sites present in the 5'-flanking region of OfCXCR4 coupled with 
      its observed regulated mRNA expression suggest that it may have an important role 
      in immune surveillance in rock bream.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Thulasitha, William Shanthakumar
AU  - Thulasitha WS
AD  - Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju 
      National University, Jeju Self-Governing Province 690-756, Republic of Korea; 
      Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing 
      Province 690-756, Republic of Korea.
FAU - Umasuthan, Navaneethaiyer
AU  - Umasuthan N
AD  - Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju 
      National University, Jeju Self-Governing Province 690-756, Republic of Korea; 
      Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing 
      Province 690-756, Republic of Korea.
FAU - Revathy, Kasthuri Saranya
AU  - Revathy KS
AD  - Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju 
      National University, Jeju Self-Governing Province 690-756, Republic of Korea.
FAU - Whang, Ilson
AU  - Whang I
AD  - Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing 
      Province 690-756, Republic of Korea.
FAU - Lee, Jehee
AU  - Lee J
AD  - Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju 
      National University, Jeju Self-Governing Province 690-756, Republic of Korea; 
      Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing 
      Province 690-756, Republic of Korea. Electronic address: jehee@jejunu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150318
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, CXCR4)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chemokine CXCL12/metabolism
MH  - Cloning, Molecular
MH  - Edwardsiella tarda/immunology
MH  - Gene Components
MH  - Gene Expression Profiling/veterinary
MH  - Head Kidney/metabolism
MH  - Lipopolysaccharides/immunology
MH  - Molecular Sequence Data
MH  - Perciformes/*genetics/*immunology/metabolism
MH  - Poly I-C/immunology
MH  - Protein Conformation
MH  - Receptors, CXCR4/*genetics/immunology/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sequence Analysis, DNA/veterinary
MH  - Sequence Homology
MH  - Streptococcus/immunology
MH  - Time Factors
OTO - NOTNLM
OT  - CXC chemokine receptor 4 (CXCR4)
OT  - Genomic structure
OT  - Immune challenge
OT  - mRNA expression profiling
EDAT- 2015/03/22 06:00
MHDA- 2016/01/29 06:00
CRDT- 2015/03/22 06:00
PHST- 2014/11/14 00:00 [received]
PHST- 2015/03/09 00:00 [revised]
PHST- 2015/03/10 00:00 [accepted]
PHST- 2015/03/22 06:00 [entrez]
PHST- 2015/03/22 06:00 [pubmed]
PHST- 2016/01/29 06:00 [medline]
AID - S1050-4648(15)00111-4 [pii]
AID - 10.1016/j.fsi.2015.03.012 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2015 Jun;44(2):471-7. doi: 10.1016/j.fsi.2015.03.012. 
      Epub 2015 Mar 18.