PMID- 25795322
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20150330
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 988
DP  - 2015 Apr 15
TI  - Multi-class mycotoxins analysis in Angelica sinensis by ultra fast liquid 
      chromatography coupled with tandem mass spectrometry.
PG  - 175-81
LID - S1570-0232(15)00114-2 [pii]
LID - 10.1016/j.jchromb.2015.02.022 [doi]
AB  - An ultra fast liquid chromatography coupled with tandem mass spectrometry 
      (UFLC-MS/MS) method was developed and validated for simultaneous analysis of 
      multi-class mycotoxins including aflatoxins (AFB1, AFB2, AFG1 and AFG2), 
      ochratoxin A (OTA), fumonisins (FB1 and FB2) and zearalanone (ZEN) in 20 batches 
      of Angelica sinensis samples collected from different markets and stores in 
      China. The eight mycotoxins were extracted and cleaned up by using QuEChERS-based 
      procedure, and then were quantified under the multiple reaction monitoring (MRM) 
      together with positive and negative ionization modes. Focusing on the 
      optimization of extraction and clean-up conditions, as well as UFLC separation 
      and MS/MS parameters of targeted analytes, the developed method expressed good 
      linearity for the eight mycotoxins within their respective linear ranges with 
      correlation coefficients all higher than 0.9974. The limits of detection (LODs) 
      and quantification (LOQs) ranged from 0.005 to 0.125 mug/kg and from 0.0625 to 
      0.25 mug/kg, respectively. Recoveries for spiked A. sinensis sample at three 
      different levels were all above 78.9% with relative standard deviations (RSDs) 
      below 6.36% for all analytes. Analysis of real samples demonstrated that two 
      visibly moldy A. sinensis samples were detected with AFB1 of 2.07 and 2.92 mug/kg, 
      and AFG1 of 2.84 and 1.53 mug/kg. The proposed quantitative method with 
      significant advantages including simple pretreatment, rapid determination and 
      high sensitivity would be the preferred candidate for the determination and 
      quantification of multi-class mycotoxin contaminants in complex matrixes, which 
      well fulfilled the maximum residue limits (MRLs) from various countries.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Liu, Qiutao
AU  - Liu Q
AD  - Pharmacy College, Liaoning Medical University, Jinzhou, Liaoning 121001, China; 
      Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & 
      Peking Union Medical College, Beijing 100193, China.
FAU - Kong, Weijun
AU  - Kong W
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & 
      Peking Union Medical College, Beijing 100193, China.
FAU - Guo, Weiying
AU  - Guo W
AD  - Pharmacy College, Liaoning Medical University, Jinzhou, Liaoning 121001, China. 
      Electronic address: wyinguo@163.com.
FAU - Yang, Meihua
AU  - Yang M
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & 
      Peking Union Medical College, Beijing 100193, China. Electronic address: 
      yangmeihua15@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150223
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Angelica sinensis/*microbiology
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - Linear Models
MH  - Mycotoxins/*analysis/*chemistry
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - A. sinensis
OT  - MRM
OT  - Multi-class mycotoxins
OT  - QuEChERS
OT  - UFLC-MS/MS
EDAT- 2015/03/22 06:00
MHDA- 2015/12/17 06:00
CRDT- 2015/03/22 06:00
PHST- 2015/01/11 00:00 [received]
PHST- 2015/02/08 00:00 [revised]
PHST- 2015/02/15 00:00 [accepted]
PHST- 2015/03/22 06:00 [entrez]
PHST- 2015/03/22 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
AID - S1570-0232(15)00114-2 [pii]
AID - 10.1016/j.jchromb.2015.02.022 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Apr 15;988:175-81. doi: 
      10.1016/j.jchromb.2015.02.022. Epub 2015 Feb 23.