PMID- 25796212 OWN - NLM STAT- MEDLINE DCOM- 20160225 LR - 20150606 IS - 1872-7492 (Electronic) IS - 0168-1702 (Linking) VI - 203 DP - 2015 May 4 TI - Pathogenicity of three type 2 porcine reproductive and respiratory syndrome virus strains in experimentally inoculated pregnant gilts. PG - 24-35 LID - S0168-1702(15)00123-9 [pii] LID - 10.1016/j.virusres.2015.03.005 [doi] AB - Mechanisms of reproductive failure resulting from infection with porcine reproductive and respiratory syndrome virus (PRRSV) are still poorly understood. Presented herein are the results of a side-by-side evaluation of the pathogenicity of three type 2 PRRSV strains in a reproductive model, from a pilot study used to develop experimental conditions and laboratory methods for a larger experiment. Pregnant gilts were experimentally infected with PRRSV at gestation day 85 or served as uninfected negative controls. After 21 days, all gilts and fetuses were necropsied. Clinical signs, litter outcome, viral load, cytokine levels, and pathology were compared from samples collected among pigs exposed to the three PRRSV strains. Based on differences in histologic lesions, and fetal weights, and numeric differences in gilt serum cytokine levels, litter outcome and virus replication in fetal tissues KS06-483 appeared less virulent than NVSL 97-7895 and KS06-72109 isolates. Levels of chemokine ligand 2 (CCL2), interferon alpha (IFNalpha), and interferon gamma (IFNgamma) were increased in PPRRSV-infected compared to non-infected gilts (0.01 > P < 0.06). Inoculation with NVSL 97-7895 induced higher levels of all three cytokines. All three PRRSV isolates were able to induce high mean viral load in individual litters, which was closely related to the proportion of PRRSV positive fetuses in the litter. Viral load in fetal samples was also positively associated with viral load at the maternal-fetal interface. All but one dead fetus were positive for PRRSV RNA, and higher concentrations of PRRSV RNA in fetal thymus increased the odds of fetal death. Our results suggest that virus replication in fetal tissues and the maternal-fetal interface, but not in other gilt tissues, are important for the outcome of reproductive PRRS. Additionally, our data indicate that umbilical lesions decreased corresponding to the use of pentobarbital sedation prior to euthanasia of pregnant gilts by captive bolt. CI - Copyright (c) 2015 Elsevier B.V. All rights reserved. FAU - Ladinig, Andrea AU - Ladinig A AD - Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N5B4, Canada. Electronic address: andrea.ladinig@vetmeduni.ac.at. FAU - Detmer, Susan E AU - Detmer SE AD - Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N5B4, Canada. Electronic address: susan.detmer@usask.ca. FAU - Clarke, Kyle AU - Clarke K AD - Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N5B4, Canada. Electronic address: kyle.clarke@usask.ca. FAU - Ashley, Carolyn AU - Ashley C AD - Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N5B4, Canada. Electronic address: carolyn.ashley@usask.ca. FAU - Rowland, Raymond R R AU - Rowland RR AD - Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, K-221 Mosier Hall, Manhattan, KS 66506-5802, USA. Electronic address: browland@vet.k-state.edu. FAU - Lunney, Joan K AU - Lunney JK AD - Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, 10300 Baltimore Blvd., Building 003, Beltsville, MD 20705, USA. Electronic address: Joan.Lunney@ARS.USDA.GOV. FAU - Harding, John C S AU - Harding JC AD - Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N5B4, Canada. Electronic address: john.harding@usask.ca. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150318 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Cytokines) SB - IM MH - Animal Experimentation MH - Animal Structures/pathology MH - Animals MH - Cytokines/blood MH - Female MH - Histocytochemistry MH - Pilot Projects MH - Porcine Reproductive and Respiratory Syndrome/*pathology/virology MH - Porcine respiratory and reproductive syndrome virus/*pathogenicity MH - Pregnancy MH - Pregnancy Complications, Infectious/pathology/*veterinary/virology MH - Swine MH - Viral Load MH - Virulence OTO - NOTNLM OT - Cytokine OT - Fetal preservation OT - PRRSV OT - Pathogenicity OT - Pregnant gilt OT - Viral load EDAT- 2015/03/23 06:00 MHDA- 2016/02/26 06:00 CRDT- 2015/03/23 06:00 PHST- 2014/09/05 00:00 [received] PHST- 2015/01/09 00:00 [revised] PHST- 2015/03/09 00:00 [accepted] PHST- 2015/03/23 06:00 [entrez] PHST- 2015/03/23 06:00 [pubmed] PHST- 2016/02/26 06:00 [medline] AID - S0168-1702(15)00123-9 [pii] AID - 10.1016/j.virusres.2015.03.005 [doi] PST - ppublish SO - Virus Res. 2015 May 4;203:24-35. doi: 10.1016/j.virusres.2015.03.005. Epub 2015 Mar 18.