PMID- 25796349
OWN - NLM
STAT- MEDLINE
DCOM- 20160215
LR  - 20181202
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 92
DP  - 2015 May
TI  - Versatile polyion complex micelles for peptide and siRNA vectorization to 
      engineer tolerogenic dendritic cells.
PG  - 216-27
LID - S0939-6411(15)00146-0 [pii]
LID - 10.1016/j.ejpb.2015.03.013 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that play a 
      critical role in maintaining the balance between immunity and tolerance and, as 
      such are a promising immunotherapy tool to induce immunity or to restore 
      tolerance. The main challenge to harness the tolerogenic properties of DCs is to 
      preserve their immature phenotype. We recently developed polyion complex 
      micelles, formulated with double hydrophilic block copolymers of poly(methacrylic 
      acid) and poly(ethylene oxide) blocks and able to entrap therapeutic molecules, 
      which did not induce DC maturation. In the current study, the intrinsic 
      destabilizing membrane properties of the polymers were used to optimize endosomal 
      escape property of the micelles in order to propose various strategies to restore 
      tolerance. On the first hand, we showed that high molecular weight (Mw) 
      copolymer-based micelles were efficient to favor the release of the 
      micelle-entrapped peptide into the endosomes, and thus to improve peptide 
      presentation by immature (i) DCs. On the second hand, we put in evidence that low 
      Mw copolymer-based micelles were able to favor the cytosolic release of 
      micelle-entrapped small interfering RNAs, dampening the DCs immunogenicity. 
      Therefore, we demonstrate the versatile use of polyionic complex micelles to 
      preserve tolerogenic properties of DCs. Altogether, our results underscored the 
      potential of such micelle-loaded iDCs as a therapeutic tool to restore tolerance 
      in autoimmune diseases.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Mebarek, Naila
AU  - Mebarek N
AD  - Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM, Equipe MACS, 8 rue 
      de l'Ecole Normale, 34296 Montpellier Cedex 5, France.
FAU - Vicente, Rita
AU  - Vicente R
AD  - Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM, Equipe MACS, 8 rue 
      de l'Ecole Normale, 34296 Montpellier Cedex 5, France; Inserm, U 1183, Universite 
      Montpellier, CHU St-Eloi, 80 avenue Augustin Fliche, 34298 Montpellier Cedex 5, 
      France.
FAU - Aubert-Pouessel, Anne
AU  - Aubert-Pouessel A
AD  - Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM, Equipe MACS, 8 rue 
      de l'Ecole Normale, 34296 Montpellier Cedex 5, France. Electronic address: 
      aaubert@univ-montp1.fr.
FAU - Quentin, Julie
AU  - Quentin J
AD  - Inserm, U 1183, Universite Montpellier, CHU St-Eloi, 80 avenue Augustin Fliche, 
      34298 Montpellier Cedex 5, France.
FAU - Mausset-Bonnefont, Anne-Laure
AU  - Mausset-Bonnefont AL
AD  - Inserm, U 1183, Universite Montpellier, CHU St-Eloi, 80 avenue Augustin Fliche, 
      34298 Montpellier Cedex 5, France.
FAU - Devoisselle, Jean-Marie
AU  - Devoisselle JM
AD  - Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM, Equipe MACS, 8 rue 
      de l'Ecole Normale, 34296 Montpellier Cedex 5, France.
FAU - Jorgensen, Christian
AU  - Jorgensen C
AD  - Inserm, U 1183, Universite Montpellier, CHU St-Eloi, 80 avenue Augustin Fliche, 
      34298 Montpellier Cedex 5, France.
FAU - Begu, Sylvie
AU  - Begu S
AD  - Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM, Equipe MACS, 8 rue 
      de l'Ecole Normale, 34296 Montpellier Cedex 5, France.
FAU - Louis-Plence, Pascale
AU  - Louis-Plence P
AD  - Inserm, U 1183, Universite Montpellier, CHU St-Eloi, 80 avenue Augustin Fliche, 
      34298 Montpellier Cedex 5, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150318
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (Micelles)
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 0 (Polymethacrylic Acids)
RN  - 0 (RNA, Small Interfering)
RN  - 25087-26-7 (polymethacrylic acid)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Dendritic Cells/*immunology
MH  - Endosomes/metabolism
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Immune Tolerance
MH  - Micelles
MH  - Molecular Weight
MH  - Peptides/*administration & dosage
MH  - Polyethylene Glycols/chemistry
MH  - Polymers/*chemistry
MH  - Polymethacrylic Acids/chemistry
MH  - RNA, Small Interfering/*administration & dosage
OTO - NOTNLM
OT  - Antigen presentation
OT  - Dendritic cells
OT  - Endosomal escape
OT  - Gene silencing
OT  - Polyion complex micelles
EDAT- 2015/03/23 06:00
MHDA- 2016/02/16 06:00
CRDT- 2015/03/23 06:00
PHST- 2014/07/10 00:00 [received]
PHST- 2015/03/06 00:00 [revised]
PHST- 2015/03/10 00:00 [accepted]
PHST- 2015/03/23 06:00 [entrez]
PHST- 2015/03/23 06:00 [pubmed]
PHST- 2016/02/16 06:00 [medline]
AID - S0939-6411(15)00146-0 [pii]
AID - 10.1016/j.ejpb.2015.03.013 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2015 May;92:216-27. doi: 10.1016/j.ejpb.2015.03.013. Epub 
      2015 Mar 18.