PMID- 25798249 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 2049-9434 (Print) IS - 2049-9442 (Electronic) IS - 2049-9434 (Linking) VI - 3 IP - 2 DP - 2015 Mar TI - Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation. PG - 242-246 AB - Due to their capacity to self-replicate or produce specific differentiated cell types, neural stem cells (NSCs) and bone marrow derived-mesenchymal stem cells (BMSCs) are potential sources for cell transplantation therapies, particularly for neural injury. However, the interaction between NSCs and BMSCs during differentiation has not yet been defined. The interaction is believed to improve the effectiveness and efficiency of cell therapy. In the present study, human NSCs and BMSCs were cultured and the Transwell co-culture system was used to observe the interplay between NSCs and BMSCs during differentiation. The results revealed that NSCs promoted BMSCs to differentiate into neurons and NSCs; whereas, BMSCs did not affect the differentiation of NSCs. Simultaneously, co-culture increased the concentration of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which are secreted by NSCs and BMSCs. The present findings suggest that co-culture of NSCs and BMSCs can promote the differentiation and this process may be modulated by BDNF and NGF. FAU - Rong, J U AU - Rong JU AD - Department of Neonatology, Chengdu Women's and Children's Central Hospital, Chongqing Medical University, Chengdu, Sichuan 610091, P.R. China. FAU - Wen, Zeng AU - Wen Z AD - Department of Neonatology, Chengdu Women's and Children's Central Hospital, Chongqing Medical University, Chengdu, Sichuan 610091, P.R. China. FAU - Rong, W U AU - Rong WU AD - Neonatal Medical Center, Huaian Maternity and Child Healthcare Hospital Affiliated to Yangzhou University Medical Academy, Huaian, Jiangsu 223002, P.R. China. FAU - Zhichun, Feng AU - Zhichun F AD - Department of Neonatology, Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command, Beijing 100700, P.R. China. LA - eng PT - Journal Article DEP - 20141217 PL - England TA - Biomed Rep JT - Biomedical reports JID - 101613227 PMC - PMC4360510 OTO - NOTNLM OT - bone marrow derived-mesenchymal stem cells OT - brain-derived neurotrophic factor OT - co-culture OT - differentiation OT - nerve growth factor OT - neural stem cells EDAT- 2015/03/24 06:00 MHDA- 2015/03/24 06:01 PMCR- 2014/12/17 CRDT- 2015/03/24 06:00 PHST- 2014/07/15 00:00 [received] PHST- 2014/12/05 00:00 [accepted] PHST- 2015/03/24 06:00 [entrez] PHST- 2015/03/24 06:00 [pubmed] PHST- 2015/03/24 06:01 [medline] PHST- 2014/12/17 00:00 [pmc-release] AID - br-03-02-0242 [pii] AID - 10.3892/br.2014.405 [doi] PST - ppublish SO - Biomed Rep. 2015 Mar;3(2):242-246. doi: 10.3892/br.2014.405. Epub 2014 Dec 17.