PMID- 25799169
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - The effects of secretion factors from umbilical cord derived mesenchymal stem 
      cells on osteogenic differentiation of mesenchymal stem cells.
PG  - e0120593
LID - 10.1371/journal.pone.0120593 [doi]
LID - e0120593
AB  - Factors synthesized by mesenchymal stem cells (MSCs) contain various growth 
      factors, cytokines, exosomes and microRNAs, which may affect the differentiation 
      abilities of MSCs. In the present study, we investigated the effects of secretion 
      factors of human umbilical cord derived mesenchymal stem cells (hUCMSCs) on 
      osteogenesis of human bone marrow derived MSCs (hBMSCs). The results showed that 
      20 mug/ml hUCMSCs secretion factors could initiate osteogenic differentiation of 
      hBMSCs without osteogenic induction medium (OIM), and the amount of calcium 
      deposit (stained by Alizarin Red) was significantly increased after the hUCMSCs 
      secretion factors treatment. Real time quantitative reverse 
      transcription-polymerase chain reaction (real time qRT-PCR) demonstrated that the 
      expression of osteogenesis-related genes including ALP, BMP2, OCN, Osterix, Col1alpha 
      and Runx2 were significantly up-regulated following hUCMSCs secretion factors 
      treatment. In addition, we found that 10 mug hUCMSCs secretion factors together 
      with 2x10(5) hBMSCs in the HA/TCP scaffolds promoted ectopic bone formation in 
      nude mice. Local application of 10 mug hUCMSCs secretion factors with 50 mul 2% 
      hyaluronic acid hydrogel and 1x10(5) rat bone marrow derived MSCs (rBMSCs) also 
      significantly enhanced the bone repair of rat calvarial bone critical defect 
      model at both 4 weeks and 8 weeks. Moreover, the group that received the hUCMSCs 
      secretion factors treatment had more cartilage and bone regeneration in the 
      defect areas than those in the control group. Taken together, these findings 
      suggested that hUCMSCs secretion factors can initiate osteogenesis of bone marrow 
      MSCs and promote bone repair. Our study indicates that hUCMSCs secretion factors 
      may be potential sources for promoting bone regeneration.
FAU - Wang, Kui-Xing
AU  - Wang KX
AD  - Ministry of Education Key Laboratory for Regenerative Medicine, School of 
      Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, 
      Hong Kong, China; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing 
      Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of 
      Hong Kong, Hong Kong, China.
FAU - Xu, Liang-Liang
AU  - Xu LL
AD  - Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health 
      Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong 
      Kong, China; Department of Orthopaedics & Traumatology, Faculty of Medicine, 
      Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China; 
      Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese 
      University of Hong Kong, Hong Kong, China.
FAU - Rui, Yun-Feng
AU  - Rui YF
AD  - Department of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast 
      University, Nanjing, China.
FAU - Huang, Shuo
AU  - Huang S
AD  - Department of Orthopaedics & Traumatology, Faculty of Medicine, Prince of Wales 
      Hospital, The Chinese University of Hong Kong, Hong Kong, China; Lui Che Woo 
      Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of 
      Hong Kong, Hong Kong, China.
FAU - Lin, Si-En
AU  - Lin SE
AD  - Department of Orthopaedics & Traumatology, Faculty of Medicine, Prince of Wales 
      Hospital, The Chinese University of Hong Kong, Hong Kong, China; Lui Che Woo 
      Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of 
      Hong Kong, Hong Kong, China.
FAU - Xiong, Jiang-Hui
AU  - Xiong JH
AD  - The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal 
      System, The Chinese University of Hong Kong Shenzhen Research Institute, 
      Shenzhen, China; State Key Laboratory of Space Medical Fundamentation and 
      Application, Astronaut Research and Training Center of China (ACC), 26 Beiqing 
      Road, 100094, Beijing, China.
FAU - Li, Ying-Hui
AU  - Li YH
AD  - The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal 
      System, The Chinese University of Hong Kong Shenzhen Research Institute, 
      Shenzhen, China; State Key Laboratory of Space Medical Fundamentation and 
      Application, Astronaut Research and Training Center of China (ACC), 26 Beiqing 
      Road, 100094, Beijing, China.
FAU - Lee, Wayne Yuk-Wai
AU  - Lee WY
AD  - Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health 
      Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong 
      Kong, China; Department of Orthopaedics & Traumatology, Faculty of Medicine, 
      Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China; 
      Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese 
      University of Hong Kong, Hong Kong, China.
FAU - Li, Gang
AU  - Li G
AD  - Ministry of Education Key Laboratory for Regenerative Medicine, School of 
      Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, 
      Hong Kong, China; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing 
      Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of 
      Hong Kong, Hong Kong, China; Department of Orthopaedics & Traumatology, Faculty 
      of Medicine, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong 
      Kong, China; Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, 
      The Chinese University of Hong Kong, Hong Kong, China; Department of 
      Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, 
      Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150323
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - *Autocrine Communication
MH  - Biomarkers
MH  - Bone Regeneration/drug effects
MH  - Cell Culture Techniques
MH  - *Cell Differentiation/drug effects
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - *Osteogenesis/drug effects
MH  - Phenotype
MH  - Rats
MH  - Umbilical Cord/*cytology
PMC - PMC4370627
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/03/24 06:00
MHDA- 2015/12/17 06:00
PMCR- 2015/03/23
CRDT- 2015/03/24 06:00
PHST- 2014/10/13 00:00 [received]
PHST- 2015/01/24 00:00 [accepted]
PHST- 2015/03/24 06:00 [entrez]
PHST- 2015/03/24 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
PHST- 2015/03/23 00:00 [pmc-release]
AID - PONE-D-14-39853 [pii]
AID - 10.1371/journal.pone.0120593 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 23;10(3):e0120593. doi: 10.1371/journal.pone.0120593. 
      eCollection 2015.