PMID- 25800019
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20150324
IS  - 1096-9888 (Electronic)
IS  - 1076-5174 (Linking)
VI  - 50
IP  - 2
DP  - 2015 Feb
TI  - Comparison of MALDI-TOF mass spectra with microsatellite length polymorphisms in 
      Candida albicans.
PG  - 371-7
LID - 10.1002/jms.3538 [doi]
AB  - Candida albicans is the most frequent yeast involved in human infections. Its 
      population structure can be divided into several genetic clades, some of which 
      have been associated with antifungal susceptibility. Therefore, detecting and 
      monitoring fungal clones in a routine laboratory setting would be a major 
      epidemiological advance. Matrix-assisted laser desorption/ionization 
      time-of-flight (MALDI-TOF) mass spectra results are now widely used as bar codes 
      to identify microorganisms in clinical microbiology laboratories. This study 
      aimed at testing MALDI-TOF mass spectra bar codes to identify clades among a set 
      of C. albicans isolates. Accordingly, 102 clinical strains were genotyped using 
      10 microsatellite markers and analyzed via MALDI-TOF mass spectrometry. The mass 
      spectra were compared with a reference spectral library including 33 
      well-characterized collection strains, using a Microflex(TM) system and 
      Biotyper(TM) software, to test the capacity of the spectrum of a given isolate to 
      match with the reference mass spectrum of an isolate from the same genetic clade. 
      Despite high confidence species identification, the spectra failed to 
      significantly match with the corresponding clade (p = 0.74). This was confirmed 
      with the MALDI-TOF spectra similarity dendrogram, in which the strains were 
      dispersed irrespective of their genetic clade. Various attempts to improve 
      intra-clade spectra recognition were unsuccessful. In conclusion, MALDI-TOF mass 
      spectra bar code analysis failed to reliably recognize genetically related C. 
      albicans isolates. Further studies are warranted to develop alternative MALDI-TOF 
      mass spectra analytical approaches to identify and monitor C. albicans clades in 
      the routine clinical laboratory.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Dhieb, C
AU  - Dhieb C
AD  - Laboratoire de Microorganismes et Biomolecules Actives, Faculte des Sciences de 
      Tunis, 2092, Tunis, Tunisia.
FAU - Normand, A C
AU  - Normand AC
FAU - L'Ollivier, C
AU  - L'Ollivier C
FAU - Gautier, M
AU  - Gautier M
FAU - Vranckx, K
AU  - Vranckx K
FAU - El Euch, D
AU  - El Euch D
FAU - Chaker, E
AU  - Chaker E
FAU - Hendrickx, M
AU  - Hendrickx M
FAU - Dalle, F
AU  - Dalle F
FAU - Sadfi, N
AU  - Sadfi N
FAU - Piarroux, R
AU  - Piarroux R
FAU - Ranque, S
AU  - Ranque S
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - Candida albicans/classification/*genetics
MH  - Candidiasis/microbiology
MH  - DNA, Fungal/*analysis/chemistry/genetics
MH  - Humans
MH  - Microsatellite Repeats/*genetics
MH  - Molecular Typing/*methods
MH  - Mycology/*methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
OTO - NOTNLM
OT  - Candida albicans
OT  - MALDI-TOF mass spectrometry
OT  - genetic clade
OT  - microsatellite typing
OT  - yeast
EDAT- 2015/03/25 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/03/25 06:00
PHST- 2014/07/18 00:00 [received]
PHST- 2014/10/30 00:00 [revised]
PHST- 2014/11/02 00:00 [accepted]
PHST- 2015/03/25 06:00 [entrez]
PHST- 2015/03/25 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1002/jms.3538 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2015 Feb;50(2):371-7. doi: 10.1002/jms.3538.