PMID- 25800056
OWN - NLM
STAT- MEDLINE
DCOM- 20150616
LR  - 20201209
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 208
IP  - 7
DP  - 2015 Mar 30
TI  - WISp39 binds phosphorylated Coronin 1B to regulate Arp2/3 localization and 
      Cofilin-dependent motility.
PG  - 961-74
LID - 10.1083/jcb.201410095 [doi]
AB  - We previously identified Waf1 Cip1 stabilizing protein 39 (WISp39) as a binding 
      partner for heat shock protein 90 (Hsp90). We now report that WISp39 has an 
      essential function in the control of directed cell migration, which requires 
      WISp39 interaction with Hsp90. WISp39 knockdown (KD) resulted in the loss of 
      directional motility of mammalian cells and profound changes in cell morphology, 
      including the loss of a single leading edge. WISp39 binds Coronin 1B, known to 
      regulate the Arp2/3 complex and Cofilin at the leading edge. WISp39 
      preferentially interacts with phosphorylated Coronin 1B, allowing it to complex 
      with Slingshot phosphatase (SSH) to dephosphorylate and activate Cofilin. WISp39 
      also regulates Arp2/3 complex localization at the leading edge. WISp39 KD-induced 
      morphological changes could be rescued by overexpression of Coronin 1B together 
      with a constitutively active Cofilin mutant. We conclude that WISp39 associates 
      with Hsp90, Coronin 1B, and SSH to regulate Cofilin activation and Arp2/3 complex 
      localization at the leading edge.
CI  - (c) 2015 Howell et al.
FAU - Howell, Michael
AU  - Howell M
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - Brickner, Howard
AU  - Brickner H
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - Delorme-Walker, Violaine D
AU  - Delorme-Walker VD
AD  - The Scripps Research Institute, La Jolla, CA 92037.
FAU - Choi, Justin
AU  - Choi J
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - Saffin, Jean-Michel
AU  - Saffin JM
AD  - Sanford Consortium for Regenerative Medicine, University of California, San 
      Diego, La Jolla, CA 92037.
FAU - Miller, Daniel
AU  - Miller D
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - Panopoulos, Andreas
AU  - Panopoulos A
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - DerMardirossian, Celine
AU  - DerMardirossian C
AD  - The Scripps Research Institute, La Jolla, CA 92037.
FAU - Fotedar, Arun
AU  - Fotedar A
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - Margolis, Robert L
AU  - Margolis RL
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
FAU - Fotedar, Rati
AU  - Fotedar R
AD  - Sanford-Burnham Medical Research Institute, La Jolla, CA 92037 
      rfotedar@sanfordburnham.org.
LA  - eng
GR  - R01 CA108947/CA/NCI NIH HHS/United States
GR  - CA108947/CA/NCI NIH HHS/United States
GR  - CA101810/CA/NCI NIH HHS/United States
GR  - R01 GM088716/GM/NIGMS NIH HHS/United States
GR  - R01 CA101810/CA/NCI NIH HHS/United States
GR  - GM088716/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150323
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actin Depolymerizing Factors)
RN  - 0 (Actin-Related Protein 2-3 Complex)
RN  - 0 (FKBPL protein, human)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 145420-64-0 (coronin proteins)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (slingshot phosphatase 2, human)
RN  - EC 5.2.1.- (Tacrolimus Binding Proteins)
RN  - EC 5.2.1.8 (Immunophilins)
SB  - IM
MH  - Actin Depolymerizing Factors/genetics/*metabolism
MH  - Actin-Related Protein 2-3 Complex/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Enzyme Activation/genetics
MH  - HEK293 Cells
MH  - HSP90 Heat-Shock Proteins/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Immunophilins/genetics/*metabolism
MH  - Microfilament Proteins/biosynthesis/*metabolism
MH  - Phosphoprotein Phosphatases
MH  - Phosphorylation
MH  - Protein Binding
MH  - RNA Interference
MH  - RNA, Small Interfering
MH  - Tacrolimus Binding Proteins
PMC - PMC4384738
EDAT- 2015/03/25 06:00
MHDA- 2015/06/17 06:00
PMCR- 2015/09/30
CRDT- 2015/03/25 06:00
PHST- 2015/03/25 06:00 [entrez]
PHST- 2015/03/25 06:00 [pubmed]
PHST- 2015/06/17 06:00 [medline]
PHST- 2015/09/30 00:00 [pmc-release]
AID - jcb.201410095 [pii]
AID - 201410095 [pii]
AID - 10.1083/jcb.201410095 [doi]
PST - ppublish
SO  - J Cell Biol. 2015 Mar 30;208(7):961-74. doi: 10.1083/jcb.201410095. Epub 2015 Mar 
      23.