PMID- 25801418
OWN - NLM
STAT- MEDLINE
DCOM- 20160414
LR  - 20181113
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Linking)
VI  - 57
IP  - 8
DP  - 2015 Aug
TI  - The Cholera Toxin B Subunit (CTB) Fused to the Porcine Arterivirus Matrix M and 
      GP5 Envelope Proteins Fails to Enhance the GP5-Specific Antibody Response in Pigs 
      Immunized with Adenovectors.
PG  - 701-8
LID - 10.1007/s12033-015-9861-6 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus 
      of the Arteriviridae family. As the current commercial vaccines are incompletely 
      protective effective against PRRSV infection, we developed a vaccine strategy 
      using replicating but non-disseminating adenovectors (rAdVs) expressing the PRRSV 
      M matrix protein in fusion with the neutralizing major epitope-carrying GP5 
      envelope protein (Roques et al. in Vet Res 44:17, 2013). Although production of 
      GP5-specific antibodies (Abs) was observed, no PRRSV-specific neutralizing Abs 
      (NAbs) were induced in pigs given the rAdVs expressing M-GP5 or M-GP5m (GP5m 
      being a mutant form of GP5). Nevertheless, partial protection was observed in the 
      M-GP5m-rAdV-inoculated pigs experimentally infected with PRRSV. Here, we 
      determined the impact of the cholera toxin B subunit (CTB, known for its adjuvant 
      effect) in fusion with the C-terminus of M-GP5m on the Ab response to PRRSV. 
      Three-week-old pigs were immunized twice both intramuscularly and intranasally at 
      3-week intervals with rAdV-expressing the green fluorescent protein (rAdV-GFP), 
      rAdV-M-GP5m, or rAdV-M-GP5m-CTB. Pigs immunized with rAdV-M-GP5m showed a high 
      level of serum GP5-specific Abs (as determined by an indirect ELISA). In 
      contrast, CTB in fusion with M-GP5m had an unexpected severe negative impact on 
      GP5-specific Ab production. PRRSV-specific NAbs could not be detected in any pigs 
      of all groups.
FAU - Roques, Elodie
AU  - Roques E
AD  - Department of Biological Sciences, University of Quebec at Montreal, Succursale 
      Centre-Ville, P.O. Box 8888, Montreal, QC, H3C 3P8, Canada.
FAU - Lessard, Martin
AU  - Lessard M
FAU - Archambault, Denis
AU  - Archambault D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - *Adenoviridae
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - Arterivirus/genetics/*immunology
MH  - Cholera Toxin/genetics/*immunology
MH  - Immunization
MH  - Swine
MH  - Viral Envelope Proteins/genetics/*immunology
MH  - Viral Matrix Proteins/genetics/*immunology
MH  - Viral Vaccines/genetics/*immunology
EDAT- 2015/03/25 06:00
MHDA- 2016/04/15 06:00
CRDT- 2015/03/25 06:00
PHST- 2015/03/25 06:00 [entrez]
PHST- 2015/03/25 06:00 [pubmed]
PHST- 2016/04/15 06:00 [medline]
AID - 10.1007/s12033-015-9861-6 [doi]
PST - ppublish
SO  - Mol Biotechnol. 2015 Aug;57(8):701-8. doi: 10.1007/s12033-015-9861-6.