PMID- 25803042
OWN - NLM
STAT- MEDLINE
DCOM- 20160407
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - T-cell activation and early gene response in dogs.
PG  - e0121169
LID - 10.1371/journal.pone.0121169 [doi]
LID - e0121169
AB  - T-cells play a crucial role in canine immunoregulation and defence against 
      invading pathogens. Proliferation is fundamental to T-cell differentiation, 
      homeostasis and immune response. Initiation of proliferation following receptor 
      mediated stimuli requires a temporally programmed gene response that can be 
      identified as immediate-early, mid- and late phases. The immediate-early response 
      genes in T-cell activation engage the cell cycle machinery and promote subsequent 
      gene activation events. Genes involved in this immediate-early response in dogs 
      are yet to be identified. The present study was undertaken to characterise the 
      early T-cell gene response in dogs to improve understanding of the genetic 
      mechanisms regulating immune function. Gene expression profiles were 
      characterised using canine gene expression microarrays and quantitative reverse 
      transcription PCR (qRT-PCR), and paired samples from eleven dogs. Significant 
      functional annotation clusters were identified following stimulation with 
      phytohemagluttinin (PHA) (5mug/ml), including the Toll-like receptor signaling 
      pathway and phosphorylation pathways. Using strict statistical criteria, 13 
      individual genes were found to be differentially expressed, nine of which have 
      ontologies that relate to proliferation and cell cycle control. These included, 
      prostaglandin-endoperoxide synthase 2 (PTGS2/COX2), early growth response 1 
      (EGR1), growth arrest and DNA damage-inducible gene (GADD45B), 
      phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1), V-FOS FBJ murine 
      osteosarcoma viral oncogene homolog (FOS), early growth response 2 (EGR2), 
      hemogen (HEMGN), polo-like kinase 2 (PLK2) and polo-like kinase 3 (PLK3). 
      Differential gene expression was re-examined using qRT-PCR, which confirmed that 
      EGR1, EGR2, PMAIP1, PTGS2, FOS and GADD45B were significantly upregulated in 
      stimulated cells and ALAS2 downregulated. PTGS2 and EGR1 showed the highest 
      levels of response in these dogs. Both of these genes are involved in cell cycle 
      regulation. This study provides a comprehensive analysis of the early T-cell gene 
      response to activation in dogs.
FAU - Mortlock, Sally-Anne
AU  - Mortlock SA
AD  - Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
FAU - Wei, Jerry
AU  - Wei J
AD  - Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia; 
      Sydney Medical School, The University of Sydney, NSW 2006, Australia.
FAU - Williamson, Peter
AU  - Williamson P
AD  - Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
LA  - eng
SI  - GEO/GSE65158
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150324
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - Dogs/*genetics/*immunology
MH  - Female
MH  - *Genes, Immediate-Early
MH  - Lymphocyte Activation/genetics/immunology
MH  - Male
MH  - Real-Time Polymerase Chain Reaction
MH  - T-Lymphocytes/*immunology/*physiology
MH  - Transcriptome
PMC - PMC4372360
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/03/25 06:00
MHDA- 2016/04/08 06:00
PMCR- 2015/03/24
CRDT- 2015/03/25 06:00
PHST- 2014/11/06 00:00 [received]
PHST- 2015/01/28 00:00 [accepted]
PHST- 2015/03/25 06:00 [entrez]
PHST- 2015/03/25 06:00 [pubmed]
PHST- 2016/04/08 06:00 [medline]
PHST- 2015/03/24 00:00 [pmc-release]
AID - PONE-D-14-50073 [pii]
AID - 10.1371/journal.pone.0121169 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 24;10(3):e0121169. doi: 10.1371/journal.pone.0121169. 
      eCollection 2015.