PMID- 25805322 OWN - NLM STAT- MEDLINE DCOM- 20160418 LR - 20151217 IS - 1096-0007 (Electronic) IS - 0014-4835 (Linking) VI - 142 DP - 2016 Jan TI - Proinflammatory status in the aqueous humor of high myopic cataract eyes. PG - 13-8 LID - S0014-4835(15)00107-4 [pii] LID - 10.1016/j.exer.2015.03.017 [doi] AB - High myopia has long been recognized as an inflammation-related disease, and high myopic eyes are thought to have a proinflammatory internal microenvironment, which might predispose to the occurrence of certain inflammation-related complications such as fibrotic capsular contraction syndrome after cataract surgery. Therefore, the purpose of this study was to detect inflammatory cytokines expressed in the aqueous humor (AH) of high myopic cataract (HMC) patients. The cytokines were screened using a RayBio Human Cytokine Antibody Array in AH samples from 15 age-related cataract (ARC) patients and 15 HMC patients. Those detected by the screening assays were verified using a Bio-Plex Suspension Array System in AH samples from 35 ARC patients and 45 HMC patients. The cytokine antibody array showed that the expression level of interleukin-1 receptor antagonist (IL-1ra) in the AH was higher in ARC than in HMC, whereas opposite trends were found for monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T-cell expressed and presumably secreted (RANTES), IL-8, platelet-derived growth factor-BB, and IL-6 (all P < 0.05). In the verification assay using the suspension cytokine array, only the expression levels of IL-1ra and MCP-1 were significantly different between the ARC and HMC groups (P = 0.014 and 0.038, respectively); these results were confirmed by western blot assays. Our results demonstrated that the expression of IL-1ra was significantly lower and the expression of MCP-1 was significantly higher in the AH of HMC than in ARC, suggestive of a proinflammatory status in the anterior chamber of HMC eyes. CI - Copyright (c) 2015 Elsevier Ltd. All rights reserved. FAU - Zhu, Xiangjia AU - Zhu X AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - Zhang, Keke AU - Zhang K AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - He, Wenwen AU - He W AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - Yang, Jin AU - Yang J AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - Sun, Xinghuai AU - Sun X AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - Jiang, Chunhui AU - Jiang C AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - Dai, Jinhui AU - Dai J AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. FAU - Lu, Yi AU - Lu Y AD - Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China. Electronic address: luyieent@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150322 PL - England TA - Exp Eye Res JT - Experimental eye research JID - 0370707 RN - 0 (Cytokines) SB - IM MH - Aged MH - Analysis of Variance MH - Aqueous Humor/*metabolism MH - Blotting, Western MH - Cataract/*metabolism MH - Cytokines/*metabolism MH - Female MH - Humans MH - Male MH - Middle Aged MH - Myopia, Degenerative/*metabolism OTO - NOTNLM OT - Aqueous humor OT - Capsular contraction syndrome OT - Cytokine OT - Fibrosis OT - High myopia OT - Inflammation OT - Interleukin-1 receptor antagonist (IL-1ra) OT - Monocyte chemoattractant protein-1 (MCP-1) EDAT- 2015/03/26 06:00 MHDA- 2016/04/19 06:00 CRDT- 2015/03/26 06:00 PHST- 2014/09/25 00:00 [received] PHST- 2015/02/19 00:00 [revised] PHST- 2015/03/20 00:00 [accepted] PHST- 2015/03/26 06:00 [entrez] PHST- 2015/03/26 06:00 [pubmed] PHST- 2016/04/19 06:00 [medline] AID - S0014-4835(15)00107-4 [pii] AID - 10.1016/j.exer.2015.03.017 [doi] PST - ppublish SO - Exp Eye Res. 2016 Jan;142:13-8. doi: 10.1016/j.exer.2015.03.017. Epub 2015 Mar 22.