PMID- 25805833
OWN - NLM
STAT- MEDLINE
DCOM- 20150806
LR  - 20200220
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 94
IP  - 5
DP  - 2015 May
TI  - Isolation of chicken astrovirus from specific pathogen-free chicken embryonated 
      eggs.
PG  - 947-54
LID - 10.3382/ps/pev086 [doi]
AB  - Astroviruses have been associated with enteric disorders in many animal species, 
      including chickens. Here, we describe the isolation, propagation, and 
      pathological characteristics of chicken astrovirus (CAstV) in specific pathogen 
      free (SPF) chicken embryonated eggs (CEE) from chickens with diarrhea and 
      runting-stunting syndrome. The CEE were inoculated via the yolk sac route. Viral 
      confirmation was carried out using PCR techniques and transmission electron 
      microscopy negative staining with ammonium molybdate. The intestinal contents 
      were screened for CAstV, and differential diagnostic testing was performed for 
      avian nephritis virus, avian rotavirus, avian reovirus, chicken parvovirus, 
      infectious bronchitis virus, and fowl adenovirus Group I to detect co-infection 
      with other infectious agents. Seven- or 14-day-old CEEs presented with 
      hemorrhages, edema, a gelatinous aspect, deformities, and dwarfism. The 
      supporting membranes did not show any alterations. Here, we have described the 
      isolation of CAstV and its pathological characteristics in SPF CEE.
CI  - (c) 2015 Poultry Science Association Inc.
FAU - Nunez, Luis Fabian N
AU  - Nunez LF
AD  - Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, 
      Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, Sao Paulo, SP, Brazil.
FAU - Parra, Silvana H Santander
AU  - Parra SH
AD  - Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, 
      Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, Sao Paulo, SP, Brazil.
FAU - Mettifogo, Elena
AU  - Mettifogo E
AD  - Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, 
      Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, Sao Paulo, SP, Brazil.
FAU - Catroxo, Marcia Helena B
AU  - Catroxo MH
AD  - Laboratory of Electron Microscopy, Center for Research and Development of Animal 
      Health, InstitutoBiologico, Av. Cons. Rodrigues Alves, 1252, 04014-002, Sao 
      Paulo, SP, Brazil.
FAU - Astolfi-Ferreira, Claudete S
AU  - Astolfi-Ferreira CS
AD  - Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, 
      Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, Sao Paulo, SP, Brazil.
FAU - Piantino Ferreira, Antonio J
AU  - Piantino Ferreira AJ
AD  - Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, 
      Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, Sao Paulo, SP, Brazil 
      ajpferr@usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150323
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
SB  - IM
MH  - Animals
MH  - Avastrovirus/*isolation & purification/ultrastructure
MH  - Chick Embryo/*virology
MH  - Polymerase Chain Reaction/methods/veterinary
MH  - Specific Pathogen-Free Organisms
MH  - Tomography, X-Ray Computed
MH  - Virus Cultivation/methods
OTO - NOTNLM
OT  - chicken astrovirus
OT  - chicken embryonated eggs
OT  - enteric virus
OT  - viral isolation
EDAT- 2015/03/26 06:00
MHDA- 2015/08/08 06:00
CRDT- 2015/03/26 06:00
PHST- 2015/01/15 00:00 [accepted]
PHST- 2015/03/26 06:00 [entrez]
PHST- 2015/03/26 06:00 [pubmed]
PHST- 2015/08/08 06:00 [medline]
AID - S0032-5791(19)32487-3 [pii]
AID - 10.3382/ps/pev086 [doi]
PST - ppublish
SO  - Poult Sci. 2015 May;94(5):947-54. doi: 10.3382/ps/pev086. Epub 2015 Mar 23.