PMID- 25807417
OWN - NLM
STAT- MEDLINE
DCOM- 20160303
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - Effects of orange juice formulation on prebiotic functionality using an in vitro 
      colonic model system.
PG  - e0121955
LID - 10.1371/journal.pone.0121955 [doi]
LID - e0121955
AB  - A three-stage continuous fermentative colonic model system was used to monitor in 
      vitro the effect of different orange juice formulations on prebiotic activity. 
      Three different juices with and without Bimuno, a GOS mixture containing 
      galactooligosaccharides (B-GOS) were assessed in terms of their ability to induce 
      a bifidogenic microbiota. The recipe development was based on incorporating 2.75g 
      B-GOS into a 250 ml serving of juice (65 degrees Brix of concentrate juice). Alongside 
      the production of B-GOS juice, a control juice--orange juice without any 
      additional Bimuno and a positive control juice, containing all the components of 
      Bimuno (glucose, galactose and lactose) in the same relative proportions with the 
      exception of B-GOS were developed. Ion Exchange Chromotography analysis was used 
      to test the maintenance of bimuno components after the production process. Data 
      showed that sterilisation had no significant effect on concentration of B-GOS and 
      simple sugars. The three juice formulations were digested under conditions 
      resembling the gastric and small intestinal environments. Main bacterial groups 
      of the faecal microbiota were evaluated throughout the colonic model study using 
      16S rRNA-based fluorescence in situ hybridization (FISH). Potential effects of 
      supplementation of the juices on microbial metabolism were studied measuring 
      short chain fatty acids (SCFAs) using gas chromatography. Furthermore, B-GOS 
      juices showed positive modulations of the microbiota composition and metabolic 
      activity. In particular, numbers of faecal bifidobacteria and lactobacilli were 
      significantly higher when B-GOS juice was fermented compared to controls. 
      Furthermore, fermentation of B-GOS juice resulted in an increase in Roseburia 
      subcluster and concomitantly increased butyrate production, which is of potential 
      benefit to the host. In conclusion, this study has shown B-GOS within orange 
      juice can have a beneficial effect on the fecal microbiota.
FAU - Costabile, Adele
AU  - Costabile A
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      United Kingdom.
FAU - Walton, Gemma E
AU  - Walton GE
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      United Kingdom.
FAU - Tzortzis, George
AU  - Tzortzis G
AD  - Clasado Research Services Ltd, Science and Technology Centre, Whiteknights, 
      Reading, United Kingdom.
FAU - Vulevic, Jelena
AU  - Vulevic J
AD  - Clasado Research Services Ltd, Science and Technology Centre, Whiteknights, 
      Reading, United Kingdom.
FAU - Charalampopoulos, Dimitris
AU  - Charalampopoulos D
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      United Kingdom.
FAU - Gibson, Glenn R
AU  - Gibson GR
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      United Kingdom.
LA  - eng
GR  - BB/H004734/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/HOO4734/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150325
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Prebiotics)
SB  - IM
MH  - Adult
MH  - *Bifidobacterium
MH  - *Citrus sinensis
MH  - Colon/*microbiology
MH  - Female
MH  - Fermentation
MH  - Humans
MH  - Male
MH  - Microbiota/*drug effects
MH  - *Models, Biological
MH  - Prebiotics/*analysis
PMC - PMC4373861
COIS- Competing Interests: The authors have the following interests: Jelena Vulevic and 
      George Tzortzis, are employed by Clasado Ltd, who provided the B-GOS product, 
      marketed as Bimuno, used within this research. There are no patents, products in 
      development or other marketed products to declare. This does not alter the 
      authors adherence to all the PLOS ONE policies on sharing data and materials.
EDAT- 2015/03/26 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/03/25
CRDT- 2015/03/26 06:00
PHST- 2014/07/30 00:00 [received]
PHST- 2015/02/06 00:00 [accepted]
PHST- 2015/03/26 06:00 [entrez]
PHST- 2015/03/26 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/03/25 00:00 [pmc-release]
AID - PONE-D-14-33813 [pii]
AID - 10.1371/journal.pone.0121955 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 25;10(3):e0121955. doi: 10.1371/journal.pone.0121955. 
      eCollection 2015.